Egr-1 Antibody (S-25) recommended for detection of Egr-1 p82 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Egr-1 Antibody (S-25): sc-101033

Egr-1 Antibody (S-25) is rated 3.7 out of 5 by 7.
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Datasheets

    • Egr-1 Antibody (S-25) is a mouse monoclonal IgG2a κ, cited in 20 publications, provided at 100 µg/ml
    • raised against a partial recombinant protein mapping within amino acids 444-544 of Egr-1 of human origin
    • recommended for detection of Egr-1 p82 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Egr-1 (S-25): sc-101033.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Egr-1 Antibody (S-25). This work is in progress.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 3 out of 5 by from It works for western blotting. It detected a band with the expected molecular mass of EGR1 in total protein extracts from HeLa cells (1:500 in 5% milk/TBS-0.1% Tween, overnight at 4C).
Date published: 2018-02-15
Rated 1 out of 5 by from Didnot work for WB No band detected in MDA-MB-231 cell line. Used the antibody at 1:1000 dilution
Date published: 2017-06-07
Rated 5 out of 5 by from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Great for WB.
Date published: 2017-01-13
Rated 5 out of 5 by from The results of western blot were great The results of western blot were great.
Date published: 2015-12-04
Rated 4 out of 5 by from This has worked well in WB&IF This has worked well in WB&IF.
Date published: 2015-10-21
Rated 5 out of 5 by from Favorable signal achieved in Raw 264 Favorable signal achieved in Raw 264.7 whole cell lysate. -SCBT QC
Date published: 2015-05-14
Rated 3 out of 5 by from Produced acceptable immunoperoxidase nuclear Produced acceptable immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-08
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