EGF Antibody (D-5): sc-374255

EGF Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Epidermal Growth Factor (EGF) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EGF antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EGF is a crucial 53-amino acid protein that plays a significant role in cell growth, proliferation, and differentiation, making EGF vital for various physiological processes, including wound healing and tissue repair. EGF is primarily located in extracellular spaces and is secreted by various cell types, where EGF binds to the EGF receptor on the cell surface, triggering a cascade of intracellular signaling pathways that promote cell division and survival. This interaction is essential for normal cellular function and has implications in cancer biology, as aberrations in EGF signaling can lead to uncontrolled cell proliferation. EGF has been detected in numerous body fluids, including urine, saliva, milk, and platelet-rich plasma, and shares 30-40% amino acid homology with other growth factors such as transforming growth factor alpha and vaccinia virus growth factor. EGF monoclonal antibody (D-5) is an invaluable tool for researchers studying the EGF signaling pathway and its role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EGF Antibody (D-5) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-EGF Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EGF Antibody (D-5) Specifications

Alternate Product Name: Epidermal growth factor (EGF)

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 993-1021

Epitope Target: EGF

Epitope Species: rat

Product Reactivity/Detection: precursor and mature EGF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for EGF Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred EGF antibody is EGF Antibody (F-9): sc-166779.