EF-1 α1/2 Antibody (G-8): sc-377439

EF-1 α1/2 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects EF-1 alpha 1/2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-EF-1 α1/2 antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The elongation factor-1 complex, consisting of EF-1 α1 and EF-1 α2, plays a crucial role in protein synthesis by facilitating the delivery of aminoacyl tRNAs to the ribosome, a process essential for translating genetic information into functional proteins. Proper functioning of EF-1 alpha ensures accurate and efficient protein synthesis, particularly important in rapidly dividing cells. EF-1 α1 is predominantly expressed in the brain, placenta, lung, liver, kidney, and pancreas, while EF-1 α2 shows high expression levels in the heart, brain, and skeletal muscle. Both isoforms localize to the nucleus and are part of the GTP-binding elongation factor family. The gene encoding EF-1 α2, located on human chromosome 20q13.3, has been implicated in ovarian cancer development, whereas the EF-1 α1 gene, found on chromosome 6Q14.1, is often recognized as an autoantigen in patients with Felty syndrome, a condition characterized by rheumatoid arthritis, splenomegaly, leukopenia, and increased infection risk.

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EF-1 α1/2 Antibody (G-8) References:

1. The role of protein elongation factor eEF1A2 in ovarian cancer.  |  Lee, JM. 2003. Reprod Biol Endocrinol. 1: 69. PMID: 14588074
2. Retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family.  |  Madsen, HO., et al. 1990. Nucleic Acids Res. 18: 1513-6. PMID: 2183196
3. Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression.  |  Scaggiante, B., et al. 2012. Br J Cancer. 106: 166-73. PMID: 22095224
4. Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment.  |  Itagaki, K., et al. 2012. J Biol Chem. 287: 16037-46. PMID: 22399298
5. Inhibition of ER stress-mediated apoptosis in macrophages by nuclear-cytoplasmic relocalization of eEF1A by the HIV-1 Nef protein.  |  Abbas, W., et al. 2012. Cell Death Dis. 3: e292. PMID: 22476100
6. Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1.  |  Lin, WS., et al. 2012. J Med Virol. 84: 871-7. PMID: 22499008
7. Molecular markers associated with nonepithelial ovarian cancer in formalin-fixed, paraffin-embedded specimens by genome wide expression profiling.  |  Vui-Kee, K., et al. 2012. Kaohsiung J Med Sci. 28: 243-50. PMID: 22531302
8. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha.  |  Uetsuki, T., et al. 1989. J Biol Chem. 264: 5791-8. PMID: 2564392
9. The primary structure of the alpha subunit of human elongation factor 1. Structural aspects of guanine-nucleotide-binding sites.  |  Brands, JH., et al. 1986. Eur J Biochem. 155: 167-71. PMID: 3512269
10. A novel 3'tRNA-derived fragment tRF-Val promotes proliferation and inhibits apoptosis by targeting EEF1A1 in gastric cancer.  |  Cui, H., et al. 2022. Cell Death Dis. 13: 471. PMID: 35585048
11. Nanoparticles (NPs)-mediated lncBCMA silencing to promote eEF1A1 ubiquitination and suppress breast cancer growth and metastasis.  |  Yang, K., et al. 2023. Acta Pharm Sin B. 13: 3489-3502. PMID: 37655325
12. KAT8-catalyzed lactylation promotes eEF1A2-mediated protein synthesis and colorectal carcinogenesis.  |  Xie, B., et al. 2024. Proc Natl Acad Sci U S A. 121: e2314128121. PMID: 38359291

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EF-1 α1/2 Antibody (G-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-EF-1 α1/2 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EF-1 α1/2 Antibody (G-8) Specifications

Alternate Product Name: EEF1A1

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 163-462

Epitope Target: EF-1 α1

Epitope Species: human

Product Reactivity/Detection: EF-1 α1 and EF-1 α2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for EF-1 α1/2 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred EF-1 α1 antibody is EF-1 α1 Antibody (CBP-KK1): sc-21758.