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EEA1 Antibody (G-4): sc-137130

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Datasheets
  • EEA1 Antibody (G-4) is a mouse monoclonal IgG1 κ EEA1 antibody, cited in 53 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of EEA1 of human origin
  • EEA1 Antibody (G-4) is recommended for detection of EEA1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-EEA1 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EEA1 Antibody (G-4) for WB and IHC(P) applications. These reagents are now offered in bundles with EEA1 Antibody (G-4) (see ordering information below).

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EEA1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects EEA1 protein of mouse, rat, human, and monkey origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EEA1 Antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EEA1, also known as early endosome antigen 1, plays a crucial role in the early endosomal pathway, which is essential for sorting and transport of endocytosed proteins. EEA1 is primarily located in the cytoplasm, where EEA1 associates with early endosomes, and proper localization is vital for regulation of membrane trafficking processes. EEA1 contains a calmodulin-binding IQ motif and a cysteine-rich finger motif, both necessary for specific localization to early endosomes. EEA1 interacts with transferrin receptor and Rab5, highlighting EEA1′s importance in the endocytic pathway. EEA1′s structure and interactions with other proteins are critical for maintaining cellular homeostasis and facilitating recycling of membrane proteins, making EEA1 a significant target for studies related to membrane dynamics and trafficking disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

EEA1 Antibody (G-4)

sc-137130
200 µg/ml
$322.00

EEA1 Antibody (G-4): m-IgG Fc BP-HRP Bundle

sc-528866
200 µg Ab; 10 µg BP
$361.00

EEA1 Antibody (G-4): m-IgGκ BP-HRP Bundle

sc-521434
200 µg Ab, 40 µg BP
$361.00

EEA1 Antibody (G-4): m-IgG1 BP-HRP Bundle

sc-543178
200 µg Ab; 20 µg BP
$361.00

EEA1 Antibody (G-4) AC

sc-137130 AC
500 µg/ml, 25% agarose
$424.00

EEA1 Antibody (G-4) HRP

sc-137130 HRP
200 µg/ml
$322.00

EEA1 Antibody (G-4) FITC

sc-137130 FITC
200 µg/ml
$336.00

EEA1 Antibody (G-4) PE

sc-137130 PE
200 µg/ml
$349.00

EEA1 Antibody (G-4) Alexa Fluor® 488

sc-137130 AF488
200 µg/ml
$364.00

EEA1 Antibody (G-4) Alexa Fluor® 546

sc-137130 AF546
200 µg/ml
$364.00

EEA1 Antibody (G-4) Alexa Fluor® 594

sc-137130 AF594
200 µg/ml
$364.00

EEA1 Antibody (G-4) Alexa Fluor® 647

sc-137130 AF647
200 µg/ml
$364.00

EEA1 Antibody (G-4) Alexa Fluor® 680

sc-137130 AF680
200 µg/ml
$364.00

EEA1 Antibody (G-4) Alexa Fluor® 790

sc-137130 AF790
200 µg/ml
$364.00

Hello I'd like to do an ELISA with an EEA1-peptide (Ala151~Val395). I am not sure if your antibody EEA1 (G-4): sc-137130 will bind the peptide. Do you have more info on the epitope? Would it be possible to get a small sample of your antibody for a test?

Asked by: KZilkens
Thank you for your question. EEA1 Antibody (G-4) is a mouse monoclonal raised against amino acids 1-300 of EEA1 of human origin. The exact epitope recognized by this antibody has not yet been determined. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-03

What is the difference between this EEA1 (G-4) clone and the discontinued polyclonal sc-33585: EA1 Antibody (H-300)?

Asked by: TinTin
EEA1 (G-4): sc-137130 is a mouse monoclonal raised against the same recombinant protein immunogen used to make polyclonal antibody sc-33585, amino acids 1-300 of EEA1 of human origin. The mouse monoclonal antibody is more specific and consistent, with published data in WB and IHC(P), and featured in several recent publications.
Answered by: Technical Support 10
Date published: 2017-01-17
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Rated 5 out of 5 by from Good for IFWe used the antibody in IF experiments, it worked very well
Date published: 2022-05-19
Rated 1 out of 5 by from NOT GREAT for IFI did Immunofluorescence staining (once PFA fixation, once combined method of fixation PFA/ MetOH) and couldn't see any signal!
Date published: 2019-02-13
Rated 1 out of 5 by from 免疫染色固定した繊維芽細胞の免疫染色に使用しました。通常通り1:400に希釈して使用して良い染色性は得られませんでした。
Date published: 2018-03-22
Rated 5 out of 5 by from good Ab for WBI tried with hippocampal culture, very strong and specfic band!
Date published: 2017-11-22
Rated 5 out of 5 by from goodI used this antibody for Immunofluorescence in 293 cell line. The result is good. But I have not done this experiment in zebrafish ,so I do not know the result is also good.
Date published: 2017-10-10
Rated 4 out of 5 by from EEA1 is ok for detecting endosome for IFI got the sample sack, this product is OK for detecting endosome for IF.
Date published: 2017-09-19
Rated 5 out of 5 by from Works great!I used this for western blotting to probe MCF7 cell lysates and it worked great!
Date published: 2017-09-04
Rated 5 out of 5 by from Works great for endosome markerVery strong and nice western blot band appeared in brain lysate (for negative control) after exosome isolation.
Date published: 2017-08-12
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EEA1 Antibody (G-4) is rated 4.5 out of 5 by 20.
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