EEA1 Antibody (G-4) recommended for detection of EEA1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA

EEA1 Antibody (G-4): sc-137130

EEA1 Antibody (G-4) is rated 4.4 out of 5 by 19.
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Datasheets

    • Anti-EEA1 Antibody (G-4) is a mouse monoclonal IgG1 (kappa light chain) EEA1 antibody provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of EEA1 of human origin
    • Anti-EEA1 Antibody (G-4) is recommended for detection of EEA1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-EEA1 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EEA1 (G-4): sc-137130.

Hello I'd like to do an ELISA with an EEA1-peptide (Ala151~Val395). I am not sure if your antibody EEA1 (G-4): sc-137130 will bind the peptide. Do you have more info on the epitope? Would it be possible to get a small sample of your antibody for a test?

Asked by: KZilkens
Thank you for your question. EEA1 Antibody (G-4) is a mouse monoclonal raised against amino acids 1-300 of EEA1 of human origin. The exact epitope recognized by this antibody has not yet been determined. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-03

What is the difference between this EEA1 (G-4) clone and the discontinued polyclonal sc-33585: EA1 Antibody (H-300)?

Asked by: TinTin
EEA1 (G-4): sc-137130 is a mouse monoclonal raised against the same recombinant protein immunogen used to make polyclonal antibody sc-33585, amino acids 1-300 of EEA1 of human origin. The mouse monoclonal antibody is more specific and consistent, with published data in WB and IHC(P), and featured in several recent publications.
Answered by: Technical Support 10
Date published: 2017-01-17
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Rated 1 out of 5 by from NOT GREAT for IF I did Immunofluorescence staining (once PFA fixation, once combined method of fixation PFA/ MetOH) and couldn't see any signal!
Date published: 2019-02-13
Rated 1 out of 5 by from 免疫染色 固定した繊維芽細胞の免疫染色に使用しました。通常通り1:400に希釈して使用して良い染色性は得られませんでした。
Date published: 2018-03-22
Rated 5 out of 5 by from good Ab for WB I tried with hippocampal culture, very strong and specfic band!
Date published: 2017-11-22
Rated 5 out of 5 by from good I used this antibody for Immunofluorescence in 293 cell line. The result is good. But I have not done this experiment in zebrafish ,so I do not know the result is also good.
Date published: 2017-10-10
Rated 4 out of 5 by from EEA1 is ok for detecting endosome for IF I got the sample sack, this product is OK for detecting endosome for IF.
Date published: 2017-09-19
Rated 5 out of 5 by from Works great! I used this for western blotting to probe MCF7 cell lysates and it worked great!
Date published: 2017-09-04
Rated 5 out of 5 by from Works great for endosome marker Very strong and nice western blot band appeared in brain lysate (for negative control) after exosome isolation.
Date published: 2017-08-12
Rated 5 out of 5 by from Great I tried 1:50 (so I haven't titrated which concentration works best) in murine dendritic cells, DC 2.4 cells. Lots of spots
Date published: 2017-07-07
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