EDG-2 Antibody (B-10): sc-515665

EDG-2 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects EDG-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EDG-2 (B-10) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. EDG-2, a member of the endothelial differentiation gene family of G protein-coupled receptors, plays a crucial role in mediating cellular responses to lysophospholipid signals, particularly lysophosphatidic acid (LPA), which is vital for processes such as cell survival, migration, and differentiation. EDG-2 expression is predominantly found in the brain, especially in white matter tracts, highlighting EDG-2′s importance in neurobiology and potential implications in neurological disorders. Additionally, anti-EDG-2 antibody (B-10) interacts with various G proteins to activate signaling pathways that influence cellular behavior, making EDG-2 a significant target for research in both developmental biology and disease mechanisms.

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EDG-2 Antibody (B-10) References:

1. Edg-2 in myelin-forming cells: isoforms, genomic mapping, and exclusion in Charcot-Marie-Tooth disease.  |  Allard, J., et al. 1999. Glia. 26: 176-85. PMID: 10384882
2. Overexpression of edg-2/vzg-1 induces apoptosis and anoikis in ovarian cancer cells in a lysophosphatidic acid-independent manner.  |  Furui, T., et al. 1999. Clin Cancer Res. 5: 4308-18. PMID: 10632375
3. A subfamily of G protein-coupled cellular receptors for lysophospholipids and lysosphingolipids.  |  Goetzl, EJ. and An, S. 1999. Adv Exp Med Biol. 469: 259-64. PMID: 10667339
4. Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6.  |  Van Brocklyn, JR., et al. 2000. Blood. 95: 2624-9. PMID: 10753843
5. Sphingosine 1-phosphate signalling in mammalian cells.  |  Pyne, S. and Pyne, NJ. 2000. Biochem J. 349: 385-402. PMID: 10880336
6. Functional comparisons of the lysophosphatidic acid receptors, LP(A1)/VZG-1/EDG-2, LP(A2)/EDG-4, and LP(A3)/EDG-7 in neuronal cell lines using a retrovirus expression system.  |  Ishii, I., et al. 2000. Mol Pharmacol. 58: 895-902. PMID: 11040035
7. Differential roles of Edg-1 and Edg-5, sphingosine 1-phosphate receptors, in the signaling pathways in C6 glioma cells.  |  Sato, K., et al. 2000. Brain Res Mol Brain Res. 85: 151-60. PMID: 11146117
8. Lysophosphatidic acid receptor-selective effects on Jurkat T cell migration through a Matrigel model basement membrane.  |  Zheng, Y., et al. 2001. J Immunol. 166: 2317-22. PMID: 11160288
9. Edg2 receptor distribution in adult rat brain.  |  Handford, EJ., et al. 2001. Neuroreport. 12: 757-60. PMID: 11277579
10. Sphingosine 1-phosphate activates Akt, nitric oxide production, and chemotaxis through a Gi protein/phosphoinositide 3-kinase pathway in endothelial cells.  |  Morales-Ruiz, M., et al. 2001. J Biol Chem. 276: 19672-7. PMID: 11278592
11. Oligodendroglial expression of Edg-2 receptor: developmental analysis and pharmacological responses to lysophosphatidic acid.  |  Stankoff, B., et al. 2002. Mol Cell Neurosci. 20: 415-28. PMID: 12139919
12. Interaction between platelets and endothelial progenitor cells via LPA-Edg-2 axis is augmented by PPAR-δ activation.  |  Han, JK., et al. 2016. J Mol Cell Cardiol. 97: 266-77. PMID: 27266389

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EDG-2 Antibody (B-10) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-EDG-2 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EDG-2 Antibody (B-10) Specifications

Alternate Product Name: EDG-2

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 186-207

Epitope Target: EDG-2

Epitope Species: human

Product Reactivity/Detection: EDG-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for EDG-2 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin