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E2F-6 Antibody (C-10) - Western Blotting - Image 308028
E2F-6 Antibody (C-10): sc-398662. Western blot analysis of E2F-6 expression in K-562 (A), Jurkat (B) and HeLa (C) nuclear extracts and Hep G2 whole cell lysate (D).
E2F-6 Antibody (C-10) - Western Blotting - Image 308028
E2F-6 Antibody (C-10): sc-398662. Western blot analysis of E2F-6 expression in K-562 (A), Jurkat (B) and HeLa (C) nuclear extracts and Hep G2 whole cell lysate (D).
E2F-6 Antibody (C-10) - Western Blotting - Image 359082
E2F-6 Antibody (C-10): sc-398662. Western blot analysis of E2F-6 expression in K-562 (A) and Jurkat (B) nuclear extracts.
E2F-6 Antibody (C-10) - Western Blotting - Image 364242
E2F-6 Antibody (C-10): sc-398662. Western blot analysis of E2F-6 expression in HL-60 (A) and JAR (B) whole cell lysates.
E2F-6 Antibody (C-10): sc-398662. Western blot analysis of E2F-6 expression in K-562 (A), Jurkat (B) and HeLa (C) nuclear extracts and Hep G2 whole cell lysate (D).

E2F-6 Antibody (C-10): sc-398662

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Datasheets
  • E2F-6 Antibody (C-10) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 232-281 of E2F-6 of human origin
  • recommended for detection of E2F-6 of human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-398662 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for E2F-6 Antibody (C-10) for WB applications. These reagents are now offered in bundles with E2F-6 Antibody (C-10) (see ordering information below).
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Additional E2F Antibodies including E2F-1, E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-8

E2F-6 Antibody (C-10) is a mouse monoclonal IgG1 antibody that detects E2F-6 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E2F-6 is a member of the E2F family of transcription factors, which play a crucial role in regulating the cell cycle and gene expression. Specifically, E2F-6 functions as a transcriptional repressor, inhibiting the expression of genes necessary for the transition from the G1 phase to the S phase of the cell cycle. This regulation is vital for maintaining proper cell proliferation and preventing uncontrolled cell growth, which can lead to tumorigenesis. E2F-6 achieves repressive function by forming complexes with other proteins, including the retinoblastoma protein (Rb), which is known to be a key player in cell cycle regulation. The interaction between E2F-6 and Rb highlights the importance of E2F-6 in the broader context of cell cycle control and cancer biology, making anti-E2F-6 antibody (C-10) an essential tool for researchers studying these critical processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

E2F-6 Antibody (C-10) References:

  1. A novel repressive E2F6 complex containing the polycomb group protein, EPC1, that interacts with EZH2 in a proliferation-specific manner.  |  Attwooll, C., et al. 2005. J Biol Chem. 280: 1199-208. PMID: 15536069
  2. A role for E2F6 in distinguishing G1/S- and G2/M-specific transcription.  |  Giangrande, PH., et al. 2004. Genes Dev. 18: 2941-51. PMID: 15574595
  3. E2F6 negatively regulates ultraviolet-induced apoptosis via modulation of BRCA1.  |  Yang, WW., et al. 2007. Cell Death Differ. 14: 807-17. PMID: 17096023
  4. E2F6 inhibits cobalt chloride-mimetic hypoxia-induced apoptosis through E2F1.  |  Yang, WW., et al. 2008. Mol Biol Cell. 19: 3691-700. PMID: 18562691
  5. EBV Nuclear Antigen 3C Mediates Regulation of E2F6 to Inhibit E2F1 Transcription and Promote Cell Proliferation.  |  Pei, Y., et al. 2016. PLoS Pathog. 12: e1005844. PMID: 27548379
  6. E2F6-mediated lncRNA CASC2 down-regulation predicts poor prognosis and promotes progression in gastric carcinoma.  |  Li, Y., et al. 2019. Life Sci. 232: 116649. PMID: 31301415
  7. CircRNA DUSP16 Knockdown Suppresses Colorectal Cancer Progression by Regulating the miR-432-5p/E2F6 Axis.  |  Wang, G. and Yang, H. 2021. Cancer Manag Res. 13: 6599-6609. PMID: 34456589
  8. Long Noncoding RNA LAMTOR5-AS1 Interference Affects MicroRNA-506-3p/E2F6-Mediated Behavior of Non-Small Cell Lung Cancer Cells.  |  Chen, G., et al. 2022. Oncol Res. 28: 945-959. PMID: 34588094
  9. MicroRNA-31 regulates TNF-α and IL-17A co-induced-endothelial cell apoptosis by repressing E2F6.  |  Fang, Z., et al. 2023. Biochem Biophys Res Commun. 666: 76-82. PMID: 37178508
  10. A hsa_circ_001726 axis regulated by E2F6 contributes to metastasis of hepatocellular carcinoma.  |  Ai, J., et al. 2024. BMC Cancer. 24: 14. PMID: 38166853

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

E2F-6 Antibody (C-10)

sc-398662
200 µg/ml
$322.00

E2F-6 Antibody (C-10): m-IgG Fc BP-HRP Bundle

sc-538492
200 µg Ab; 10 µg BP
$361.00

E2F-6 Antibody (C-10): m-IgGκ BP-HRP Bundle

sc-536117
200 µg Ab; 40 µg BP
$361.00

E2F-6 Antibody (C-10): m-IgG1 BP-HRP Bundle

sc-545729
200 µg Ab; 20 µg BP
$361.00

E2F-6 Antibody (C-10) X

sc-398662 X
200 µg/0.1 ml
$322.00

What is the recommended starting dilution for Western blot for sc-398662: E2F-6 (C-10) monoclonal antibody?

Asked by: jenniferc
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Clear band in WB in KClear band in WB in K-562, Jurkat and HeLa nuclear extracts and Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-08-16
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E2F-6 Antibody (C-10) is rated 5.0 out of 5 by 1.
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