E2F-6 Antibody (TFE61): sc-53273
- E2F-6 Antibody (TFE61) is a mouse monoclonal IgG1 κ E2F-6 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against recombinant human E2F-6
- E2F-6 Antibody (TFE61) is recommended for detection of E2F-6 of human origin by WB and IP
- Anti-E2F-6 Antibody (TFE61) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for E2F-6 Antibody (TFE61). This work is in progress.
E2F-6 Antibody (TFE61) is a mouse monoclonal IgG1 kappa light chain antibody that detects E2F-6 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-E2F-6 antibody (TFE61) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2F-6 protein plays a crucial role in regulating gene expression during cell cycle, particularly in transitioning from G1 phase to S phase, where DNA synthesis occurs. This regulation maintains proper cell proliferation and prevents uncontrolled growth, which can lead to tumorigenesis. E2F-6 functions as a transcriptional repressor, interacting with other proteins to inhibit expression of genes necessary for cell cycle progression. Formation of complexes with other E2F family members and DP-1 protein highlights E2F-6′s importance in cell cycle regulation network. Understanding E2F-6 function and interactions remains essential for elucidating mechanisms underlying cell cycle control and potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E2F-6 Antibody (TFE61) References:
- A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F. | Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
- E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1. | Kikuchi, J., et al. 2007. Stem Cells. 25: 2439-47. PMID: 17600109
- The E2F transcription factor is a cellular target for the RB protein. | Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
- The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein. | Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
- E2F6 protein levels modulate drug induced apoptosis in cardiomyocytes. | Major, JL., et al. 2017. Cell Signal. 40: 230-238. PMID: 28964969
- Predictive role of E2F6 in cancer prognosis and responses of immunotherapy. | Gong, C., et al. 2024. Int Immunopharmacol. 127: 111302. PMID: 38071912
- E2F-4, a new member of the E2F transcription factor family, interacts with p107. | Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
- E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo. | Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
- Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members. | Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
- Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. | Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
- E2F-6, a member of the E2F family that can behave as a transcriptional repressor. | Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179
- E2F-6: a novel member of the E2F family is an inhibitor of E2F-dependent transcription. | Cartwright, P., et al. 1998. Oncogene. 17: 611-23. PMID: 9704927
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2F-6 Antibody (TFE61) | sc-53273 | 200 µg/ml | $322.00 | |||
E2F-6 Antibody (TFE61) AC | sc-53273 AC | 500 µg/ml, 25% agarose | $424.00 | |||
E2F-6 Antibody (TFE61) HRP | sc-53273 HRP | 200 µg/ml | $322.00 | |||
E2F-6 Antibody (TFE61) FITC | sc-53273 FITC | 200 µg/ml | $336.00 | |||
E2F-6 Antibody (TFE61) PE | sc-53273 PE | 200 µg/ml | $349.00 | |||
E2F-6 Antibody (TFE61) Alexa Fluor® 488 | sc-53273 AF488 | 200 µg/ml | $364.00 | |||
E2F-6 Antibody (TFE61) Alexa Fluor® 546 | sc-53273 AF546 | 200 µg/ml | $364.00 | |||
E2F-6 Antibody (TFE61) Alexa Fluor® 594 | sc-53273 AF594 | 200 µg/ml | $364.00 | |||
E2F-6 Antibody (TFE61) Alexa Fluor® 647 | sc-53273 AF647 | 200 µg/ml | $364.00 | |||
E2F-6 Antibody (TFE61) Alexa Fluor® 680 | sc-53273 AF680 | 200 µg/ml | $364.00 | |||
E2F-6 Antibody (TFE61) Alexa Fluor® 790 | sc-53273 AF790 | 200 µg/ml | $364.00 |