E2F-4 Antibody (WUF3): sc-56670

E2F-4 Antibody (WUF3) is a mouse monoclonal IgG1 antibody that detects E2F-4 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). E2F-4 is a crucial member of the E2F family of transcription factors, which play a significant role in regulating the cell cycle, particularly the transition from G1 phase to S phase. Anti-E2F-4 antibody (WUF3) recognizes protein complexes essential for proper cell proliferation and differentiation, as dysregulation of E2F activity can lead to uncontrolled cell growth and is often implicated in various cancers. E2F-4 functions by forming heterodimers with DP-1 protein, creating an active transcriptional regulatory complex that binds to specific DNA sequences to activate or repress target genes involved in cell cycle progression. Precise control of E2F-4 activity ensures cells only proceed to DNA synthesis when conditions are favorable, thereby maintaining genomic integrity. E2F-4 monoclonal antibody (WUF3) serves as an invaluable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying tumorigenesis.

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E2F-4 Antibody (WUF3) References:

1. Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes.  |  Furukawa, Y., et al. 1999. Oncogene. 18: 2003-14. PMID: 10208422
2. Effect of naturally occurring E2F-4 alterations on transcriptional activation and proliferation in transfected cells.  |  Takashima, H., et al. 2001. Lab Invest. 81: 1565-73. PMID: 11706064
3. E2F4 deficiency promotes drug-induced apoptosis.  |  Ma, Y., et al. 2004. Cancer Biol Ther. 3: 1262-9. PMID: 15611646
4. In vivo genome-wide binding of Id2 to E2F4 target genes as part of a reversible program in mice liver.  |  Ferrer-Vicens, I., et al. 2014. Cell Mol Life Sci. 71: 3583-97. PMID: 24573694
5. The E2F4/p130 Repressor Complex Cooperates with Oncogenic ΔNp73α To Inhibit Gene Expression in Human Papillomavirus 38 E6/E7-Transformed Keratinocytes and in Cancer Cells.  |  Taverniti, V., et al. 2023. mSphere. 8: e0005623. PMID: 36883841
6. Enhancer-driven transcription of MCM8 by E2F4 promotes ATR pathway activation and glioma stem cell characteristics.  |  Sun, YM., et al. 2023. Hereditas. 160: 29. PMID: 37349788
7. Subcellular localization of nucleolar protein 14 and its proliferative function mediated by miR-17-5p and E2F4 in pancreatic cancer.  |  Du, YX., et al. 2023. Aging (Albany NY). 15: 7308-7323. PMID: 37506248
8. NUCB2 promotes hepatocellular carcinoma cell growth and metastasis by activating the E2F4/PTGR1 axis.  |  Wang, Y., et al. 2024. Int J Biol Sci. 20: 4767-4780. PMID: 39309426
9. Functional interaction between E2F-4 and p130: evidence for distinct mechanisms underlying growth suppression by different retinoblastoma protein family members.  |  Vairo, G., et al. 1995. Genes Dev. 9: 869-81. PMID: 7705662
10. Mutations of E2F-4 trinucleotide repeats in colorectal cancer with microsatellite instability.  |  Yoshitaka, T., et al. 1996. Biochem Biophys Res Commun. 227: 553-7. PMID: 8878551

The preferred E2F-4 antibody is E2F-4 Antibody (RK-13): sc-511.