E2F-4 Antibody (D-7): sc-398543

E2F-4 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects E2F-4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-E2F-4 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2F-4 plays a crucial role in regulating the cell cycle, particularly in the transition from G1 phase to S phase, by controlling the expression of genes necessary for DNA synthesis and cell proliferation. This regulation maintains proper cell growth and division, and dysregulation of E2F-4 can lead to uncontrolled cell proliferation, a hallmark of cancer. E2F-4 functions as a transcription factor that forms heterodimers with DP-1, creating an active E2F transcriptional regulatory complex that binds to specific DNA sequences to activate or repress target genes. The intricate balance of E2F-4 activity is essential for normal cellular function, and E2F-4′s interaction with the retinoblastoma protein (Rb) further underscores its importance in cell cycle control, as Rb′s inactivation can lead to aberrant E2F activity and contribute to tumorigenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2F-4 Antibody (D-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-E2F-4 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2F-4 Antibody (D-7) Specifications

Alternate Product Name: E2F-4

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 385-413

Epitope Target: E2F-4

Epitope Species: human

Product Reactivity/Detection: E2F-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for E2F-4 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred E2F-4 antibody is E2F-4 Antibody (RK-13): sc-511.