E2F-4 Antibody (RK-13): sc-511

E2F-4 Antibody (RK-13) is a mouse monoclonal IgG2a kappa light chain antibody that detects E2F-4 protein of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). E2F-4 (RK-13) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. E2F-4 protein plays a crucial role in regulating the cell cycle, particularly in the transition from the G1 phase to the S phase, by controlling the expression of genes necessary for DNA synthesis and cell proliferation. This regulation is vital for maintaining proper cell growth and division, as dysregulation can lead to uncontrolled cell proliferation and contribute to tumorigenesis. E2F-4 functions as a transcription factor that forms heterodimers with the DP-1 protein, creating an active E2F transcriptional regulatory complex that influences the expression of target genes. The importance of E2F-4 in cell cycle regulation underscores its potential as a therapeutic target in cancer treatment, where restoring normal E2F activity could help reinstate proper cell cycle control.

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E2F-4 Antibody (RK-13) References:

1. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
2. Interplay between the retinoblastoma related pRb2/p130 and E2F-4 and -5 in relation to JCV-TAg.  |  Caracciolo, V., et al. 2007. J Cell Physiol. 212: 96-104. PMID: 17385710
3. The E2F transcription factor is a cellular target for the RB protein.  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
4. The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein.  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
5. Anti-apoptotic function of the E2F transcription factor 4 (E2F4)/p130, a member of retinoblastoma gene family in cardiac myocytes.  |  Dingar, D., et al. 2012. J Mol Cell Cardiol. 53: 820-8. PMID: 22985930
6. E2F4DN Transgenic Mice: A Tool for the Evaluation of E2F4 as a Therapeutic Target in Neuropathology and Brain Aging.  |  Ramón-Landreau, M., et al. 2022. Int J Mol Sci. 23: PMID: 36292945
7. Transcriptional induction of NF-κB-inducing kinase by E2F4/5 facilitates collective invasion of GBM cells.  |  Pflug, KM., et al. 2023. Sci Rep. 13: 13093. PMID: 37567906
8. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
9. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
10. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
11. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
12. E2F-6, a member of the E2F family that can behave as a transcriptional repressor.  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2F-4 Antibody (RK-13) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-E2F-4 Antibody (RK-13) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2F-4 Antibody (RK-13) Specifications

Alternate Product Name: E2F-4

Product Clone Name: RK-13

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 108-300

Epitope Target: E2F-4

Epitope Species: human

Product Reactivity/Detection: E2F-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for E2F-4 Antibody (RK-13): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent