dystrophin Antibody (MANDYS8): sc-58754

Dystrophin Antibody (MANDYS8) is a mouse monoclonal IgG2b kappa light chain antibody that detects dystrophin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-dystrophin antibody (MANDYS8) is available as the non-conjugated form. Dystrophin plays a crucial role in the dystrophin-glycoprotein complex (DGC), which serves as a vital link between the F-actin cytoskeleton within muscle fibers and the surrounding extracellular matrix, thereby maintaining cellular integrity and muscle function. Dystrophin′s importance is underscored by involvement in muscle contraction and protection against mechanical stress; a deficiency in dystrophin is a primary contributor to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene spans 2.4 megabases, comprising over 80 exons and producing a 14 kb mRNA transcript that is regulated by at least eight independent tissue-specific promoters and two poly A sites. Notably, dystrophin mRNA can undergo differential splicing, resulting in a diverse array of transcripts that encode various protein isoforms. Dystrophin constitutes approximately 0.002% of total striated muscle protein and is localized to triadic junctions in skeletal muscle, where dystrophin is believed to play a significant role in calcium ion homeostasis and force transmission, highlighting essential function in muscle physiology and pathology.

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dystrophin Antibody (MANDYS8) References:

1. Dystrophin, its interactions with other proteins, and implications for muscular dystrophy.  |  Ervasti, JM. 2007. Biochim Biophys Acta. 1772: 108-17. PMID: 16829057
2. Treatment of dystrophin cardiomyopathies.  |  Finsterer, J. and Cripe, L. 2014. Nat Rev Cardiol. 11: 168-79. PMID: 24419258
3. Effect of Ataluren on dystrophin mutations.  |  Berger, J., et al. 2020. J Cell Mol Med. 24: 6680-6689. PMID: 32343037
4. Multiomic Approaches to Uncover the Complexities of Dystrophin-Associated Cardiomyopathy.  |  Gowran, A., et al. 2021. Int J Mol Sci. 22: PMID: 34445659
5. Nuclear Small Dystrophin Isoforms during Muscle Differentiation.  |  Donandt, T., et al. 2023. Life (Basel). 13: PMID: 37374149
6. The unconditioned fear response in vertebrates deficient in dystrophin.  |  Gharibi, S., et al. 2024. Prog Neurobiol. 235: 102590. PMID: 38484964
7. The structural and functional diversity of dystrophin.  |  Ahn, AH. and Kunkel, LM. 1993. Nat Genet. 3: 283-91. PMID: 7981747
8. Dystrophin and dystrophin-related proteins: a review of protein and RNA studies.  |  Love, DR., et al. 1993. Neuromuscul Disord. 3: 5-21. PMID: 8329888
9. Dystrophin as a mechanochemical transducer in skeletal muscle.  |  Brown, SC. and Lucy, JA. 1993. Bioessays. 15: 413-9. PMID: 8357344
10. Phosphorylation of the carboxyl-terminal region of dystrophin.  |  Michalak, M., et al. 1996. Biochem Cell Biol. 74: 431-7. PMID: 8960349

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dystrophin Antibody (MANDYS8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-dystrophin Antibody (MANDYS8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dystrophin Antibody (MANDYS8) Specifications

Alternate Product Name: dystrophin

Product Clone Name: MANDYS8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 816-1749

Epitope Target: dystrophin

Epitope Species: human

Product Reactivity/Detection: dystrophin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for dystrophin Antibody (MANDYS8): N/A

The preferred dystrophin antibody is dystrophin Antibody (7A10): sc-47760.