dystrophin Antibody (4C7): sc-33697
- dystrophin Antibody (4C7) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1-68 of human recombinant dystrophin
- Anti-dystrophin Antibody (4C7) is recommended for detection of dystrophin of mouse, rat and human origin by WB, IP and IF
- Anti-dystrophin Antibody (4C7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for dystrophin Antibody (4C7) for WB applications. These reagents are now offered in bundles with dystrophin Antibody (4C7) (see ordering information below).
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Dystrophin Antibody (4C7) is a mouse monoclonal IgG2b kappa light chain antibody that detects dystrophin in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Anti-dystrophin antibody (4C7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dystrophin plays a crucial role in the dystrophin-glycoprotein complex (DGC), which serves as a vital link between the F-actin cytoskeleton within muscle fibers and the extracellular matrix, thereby maintaining cellular integrity and facilitating force transmission during muscle contraction. Dystrophin′s importance is underscored by its association with Duchenne (DMD) and Becker (BMD) muscular dystrophies, conditions that arise from dystrophin deficiency. The human dystrophin gene spans 2.4 megabases with over 80 exons, producing a 14 kb mRNA that is regulated by at least eight independent tissue-specific promoters and two polyadenylation sites. Dystrophin mRNA can undergo differential splicing, resulting in a diverse array of transcripts that encode various protein isoforms. Dystrophin constitutes approximately 0.002% of total striated muscle protein and is primarily localized at triadic junctions in skeletal muscle, where dystrophin is believed to play a significant role in calcium ion homeostasis, further emphasizing dystrophin′s critical function in muscle physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
dystrophin Antibody (4C7) References:
- Function and genetics of dystrophin and dystrophin-related proteins in muscle. | Blake, DJ., et al. 2002. Physiol Rev. 82: 291-329. PMID: 11917091
- Dystrophin and mutations: one gene, several proteins, multiple phenotypes. | Muntoni, F., et al. 2003. Lancet Neurol. 2: 731-40. PMID: 14636778
- Cell cycle and dystrophin dysregulation in GIST. | Wang, Y. and Fletcher, JA. 2015. Cell Cycle. 14: 2713-4. PMID: 26101897
- Dystrophin-Deficient Cardiomyopathy. | Kamdar, F. and Garry, DJ. 2016. J Am Coll Cardiol. 67: 2533-46. PMID: 27230049
- Connecting enterovirus infection to dystrophin dysfunction in dilated cardiomyopathy. | Wang, Q. and Wehrens, XH. 2016. Ann Transl Med. 4: S23. PMID: 27867991
- Dystrophin Dp71 and the Neuropathophysiology of Duchenne Muscular Dystrophy. | Naidoo, M. and Anthony, K. 2020. Mol Neurobiol. 57: 1748-1767. PMID: 31836945
- The DMD gene and therapeutic approaches to restore dystrophin. | Fortunato, F., et al. 2021. Neuromuscul Disord. 31: 1013-1020. PMID: 34736624
- Dystrophin myonuclear domain restoration governs treatment efficacy in dystrophic muscle. | Morin, A., et al. 2023. Proc Natl Acad Sci U S A. 120: e2206324120. PMID: 36595689
- Dystrophin genetic variants and autism. | Passos-Bueno, MR., et al. 2022. Discov Ment Health. 2: 4. PMID: 37861890
- Dystrophin- and Utrophin-Based Therapeutic Approaches for Treatment of Duchenne Muscular Dystrophy: A Comparative Review. | Szwec, S., et al. 2024. BioDrugs. 38: 95-119. PMID: 37917377
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dystrophin Antibody (4C7) | sc-33697 | 200 µg/ml | $322.00 | |||
dystrophin Antibody (4C7): m-IgG Fc BP-HRP Bundle | sc-525545 | 200 µg Ab; 10 µg BP | $361.00 | |||
dystrophin Antibody (4C7): m-IgG2b BP-HRP Bundle | sc-549578 | 200 µg Ab; 10 µg BP | $361.00 | |||
dystrophin Antibody (4C7) AC | sc-33697 AC | 500 µg/ml, 25% agarose | $424.00 | |||
dystrophin Antibody (4C7) HRP | sc-33697 HRP | 200 µg/ml | $322.00 | |||
dystrophin Antibody (4C7) FITC | sc-33697 FITC | 200 µg/ml | $336.00 | |||
dystrophin Antibody (4C7) PE | sc-33697 PE | 200 µg/ml | $349.00 | |||
dystrophin Antibody (4C7) Alexa Fluor® 488 | sc-33697 AF488 | 200 µg/ml | $364.00 | |||
dystrophin Antibody (4C7) Alexa Fluor® 546 | sc-33697 AF546 | 200 µg/ml | $364.00 | |||
dystrophin Antibody (4C7) Alexa Fluor® 594 | sc-33697 AF594 | 200 µg/ml | $364.00 | |||
dystrophin Antibody (4C7) Alexa Fluor® 647 | sc-33697 AF647 | 200 µg/ml | $364.00 | |||
dystrophin Antibody (4C7) Alexa Fluor® 680 | sc-33697 AF680 | 200 µg/ml | $364.00 | |||
dystrophin Antibody (4C7) Alexa Fluor® 790 | sc-33697 AF790 | 200 µg/ml | $364.00 | |||
dystrophin Antibody (4C7) B | sc-33697 B | 200 µg/ml | $326.00 |