Dynein IC1/2, cytosolic Antibody (74-1): sc-13524

Dynein IC1/2, cytosolic Antibody (74-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects the Dynein IC1/2, cytosolic protein of broad species origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Dynein IC1/2, cytosolic (74-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dyneins are essential multisubunit ATPases that play a critical role in cellular transport by interacting with microtubules to facilitate the movement of cellular cargo toward the cell′s center. The Dynein IC1 and IC2 proteins are integral components of the cytoplasmic dynein complex, which consists of approximately 12 subunits, including two heavy chains and two intermediate chains that anchor dynein to its cargo. This complex is vital for various cellular functions, such as organelle transport and centrosome assembly, which are crucial for maintaining cellular integrity and function. The carboxy terminus of dynein is particularly important for microtubule-dependent motility and is highly conserved across species, while the amino terminal regions exhibit more variability, allowing for functional diversity. Additionally, dynein activity is regulated by several proteins, including dynactin, LIS1, and NudEL (NudE-like), which are essential for the proper functioning of dynein motors in cellular processes.

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Dynein IC1/2, cytosolic Antibody (74-1) References:

1. Cytoplasmic dynein functions as a gear in response to load.  |  Mallik, R., et al. 2004. Nature. 427: 649-52. PMID: 14961123
2. Cytoplasmic dynein nucleates microtubules to organize them into radial arrays in vivo.  |  Malikov, V., et al. 2004. Mol Biol Cell. 15: 2742-9. PMID: 15047865
3. The dynein heavy chain family.  |  Asai, DJ. and Wilkes, DE. 2004. J Eukaryot Microbiol. 51: 23-9. PMID: 15068262
4. High speed sliding of axonemal microtubules produced by outer arm dynein.  |  Seetharam, RN. and Satir, P. 2005. Cell Motil Cytoskeleton. 60: 96-103. PMID: 15605357
5. The offloading model for dynein function: differential function of motor subunits.  |  Lee, WL., et al. 2005. J Cell Biol. 168: 201-7. PMID: 15642746
6. Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments.  |  He, Y., et al. 2005. J Cell Biol. 168: 697-703. PMID: 15728192
7. NudEL targets dynein to microtubule ends through LIS1.  |  Li, J., et al. 2005. Nat Cell Biol. 7: 686-90. PMID: 15965467
8. Cytoplasmic dynein nomenclature.  |  Pfister, KK., et al. 2005. J Cell Biol. 171: 411-3. PMID: 16260502
9. Dynein motility: four heads are better than two.  |  McGrath, JL. 2005. Curr Biol. 15: R970-2. PMID: 16332531
10. Genetic analysis of the cytoplasmic dynein subunit families.  |  Pfister, KK., et al. 2006. PLoS Genet. 2: e1. PMID: 16440056

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dynein IC1/2, cytosolic Antibody (74-1) has 77 citations in a variety of scientific publications.

Primary antibodies like Anti-Dynein IC1/2, cytosolic Antibody (74-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dynein IC1/2, cytosolic Antibody (74-1) Specifications

Alternate Product Name: DYNC1I1

Product Clone Name: 74-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Dynein

Epitope Species: bovine

Product Reactivity/Detection: intermediate chains 1 and 2 of cytosolic Dynein

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 77 citations in various scientific publications

Available Conjugates/Forms for Dynein IC1/2, cytosolic Antibody (74-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin