DUOX2 Antibody (E-8): sc-398681

DUOX2 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects DUOX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DUOX2 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DUOX2 protein, also known as NADPH thyroid oxidase 2, plays a critical role in generating reactive oxygen species, particularly hydrogen peroxide, which is essential for thyroid hormone biosynthesis and antimicrobial defense in mucosal tissues. DUOX2 is primarily localized to the apical membrane of epithelial cells, including those in the thyroid, trachea, colon, and duodenum, where DUOX2 functions alongside DUOX1 to produce hydrogen peroxide necessary for the activity of thyroid peroxidase and lactoperoxidase. Proper functioning of DUOX2 is vital, as defects in the gene encoding DUOX2 can lead to congenital hypothyroidism, a condition marked by insufficient hydrogen peroxide production in the thyroid gland, ultimately affecting thyroid hormone levels and overall metabolic regulation.

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DUOX2 Antibody (E-8) References:

1. Dual oxidases represent novel hydrogen peroxide sources supporting mucosal surface host defense.  |  Geiszt, M., et al. 2003. FASEB J. 17: 1502-4. PMID: 12824283
2. Identification of a novel partner of duox: EFP1, a thioredoxin-related protein.  |  Wang, D., et al. 2005. J Biol Chem. 280: 3096-103. PMID: 15561711
3. Dual oxidase2 is expressed all along the digestive tract.  |  El Hassani, RA., et al. 2005. Am J Physiol Gastrointest Liver Physiol. 288: G933-42. PMID: 15591162
4. Dual oxidase-2 has an intrinsic Ca2+-dependent H2O2-generating activity.  |  Ameziane-El-Hassani, R., et al. 2005. J Biol Chem. 280: 30046-54. PMID: 15972824
5. Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium.  |  Harper, RW., et al. 2005. FEBS Lett. 579: 4911-7. PMID: 16111680
6. Persistent mild hypothyroidism associated with novel sequence variants of the DUOX2 gene in two siblings.  |  Vigone, MC., et al. 2005. Hum Mutat. 26: 395. PMID: 16134168
7. DUOX2 and DUOXA2 Variants Confer Susceptibility to Thyroid Dysgenesis and Gland-in-situ With Congenital Hypothyroidism.  |  Wang, F., et al. 2020. Front Endocrinol (Lausanne). 11: 237. PMID: 32425884
8. DUOX2 variants associate with preclinical disturbances in microbiota-immune homeostasis and increased inflammatory bowel disease risk.  |  Grasberger, H., et al. 2021. J Clin Invest. 131: PMID: 33651715
9. DUOX2 As a Potential Prognostic Marker which Promotes Cell Motility and Proliferation in Pancreatic Cancer.  |  Cao, M., et al. 2021. Biomed Res Int. 2021: 6530298. PMID: 33748270
10. Correlation of DUOX2 residual enzymatic activity with phenotype in congenital hypothyroidism caused by biallelic DUOX2 defects.  |  Sun, F., et al. 2021. Clin Genet. 100: 713-721. PMID: 34564849
11. Unique DUOX2+ACE2+ small cholangiocytes are pathogenic targets for primary biliary cholangitis.  |  Li, X., et al. 2023. Nat Commun. 14: 29. PMID: 36759512
12. Molecular and Clinical Features of Congenital Hypothyroidism Due to Multiple DUOX2 Variants.  |  Uehara, E., et al. 2024. Thyroid. 34: 827-836. PMID: 38757580

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DUOX2 Antibody (E-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-DUOX2 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DUOX2 Antibody (E-8) Specifications

Alternate Product Name: DUOX2

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 626-680

Epitope Target: DUOX2

Epitope Species: human

Product Reactivity/Detection: DUOX2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for DUOX2 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin