DTYMK Antibody (B-8): sc-365925
- DTYMK Antibody (B-8) is a mouse monoclonal IgG2a κ DTYMK antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-212 representing full length DTYMK of human origin
- DTYMK Antibody (B-8) is recommended for detection of DTYMK of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DTYMK Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DTYMK Antibody (B-8) for WB applications. These reagents are now offered in bundles with DTYMK Antibody (B-8) (see ordering information below).
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SEE ALSO...
DTYMK Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects DTYMK of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DTYMK (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DTYMK, also known as CDC8, TMPK, TYMK, or dTMP kinase, is a 212 amino acid enzyme that plays a crucial role in pyrimidine metabolism by catalyzing the ATP-dependent conversion of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP). This conversion is vital as dTDP serves as one of the four nucleotides necessary for DNA synthesis, thereby making DTYMK essential for cell cycle progression and growth. Notably, DTYMK expression peaks during the S phase of the cell cycle, underscoring its importance in DNA replication and cellular proliferation. The ability of DTYMK to regulate nucleotide availability directly impacts cellular functions, including DNA repair and replication, which are critical for maintaining genomic stability and preventing diseases such as cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DTYMK Antibody (B-8) References:
- Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinases. | Van Rompay, AR., et al. 2000. Pharmacol Ther. 87: 189-98. PMID: 11008000
- Structures of human thymidylate kinase in complex with prodrugs: implications for the structure-based design of novel compounds. | Ostermann, N., et al. 2003. Biochemistry. 42: 2568-77. PMID: 12614151
- Construction and complementation of in-frame deletions of the essential Escherichia coli thymidylate kinase gene. | Chaperon, DN. 2006. Appl Environ Microbiol. 72: 1288-94. PMID: 16461678
- Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. | Su, JY. and Sclafani, RA. 1991. Nucleic Acids Res. 19: 823-7. PMID: 2017365
- Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver. | Cummins, RR. and Balinsky, D. 1980. Cancer Res. 40: 1235-9. PMID: 6244089
- Human dTMP kinase: gene expression and enzymatic activity coinciding with cell cycle progression and cell growth. | Huang, SH., et al. 1994. DNA Cell Biol. 13: 461-71. PMID: 8024690
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DTYMK Antibody (B-8) | sc-365925 | 200 µg/ml | $322.00 | |||
DTYMK Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-529513 | 200 µg Ab; 10 µg BP | $361.00 | |||
DTYMK Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-522424 | 200 µg Ab, 40 µg BP | $361.00 | |||
DTYMK Antibody (B-8): m-IgG2a BP-HRP Bundle | sc-547322 | 200 µg Ab; 10 µg BP | $361.00 | |||
DTYMK Antibody (B-8) AC | sc-365925 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DTYMK Antibody (B-8) HRP | sc-365925 HRP | 200 µg/ml | $322.00 | |||
DTYMK Antibody (B-8) FITC | sc-365925 FITC | 200 µg/ml | $336.00 | |||
DTYMK Antibody (B-8) PE | sc-365925 PE | 200 µg/ml | $349.00 | |||
DTYMK Antibody (B-8) Alexa Fluor® 488 | sc-365925 AF488 | 200 µg/ml | $364.00 | |||
DTYMK Antibody (B-8) Alexa Fluor® 546 | sc-365925 AF546 | 200 µg/ml | $364.00 | |||
DTYMK Antibody (B-8) Alexa Fluor® 594 | sc-365925 AF594 | 200 µg/ml | $364.00 | |||
DTYMK Antibody (B-8) Alexa Fluor® 647 | sc-365925 AF647 | 200 µg/ml | $364.00 | |||
DTYMK Antibody (B-8) Alexa Fluor® 680 | sc-365925 AF680 | 200 µg/ml | $364.00 | |||
DTYMK Antibody (B-8) Alexa Fluor® 790 | sc-365925 AF790 | 200 µg/ml | $364.00 |