DsRed Antibody (E-8): sc-390909

DsRed Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects DsRed protein in non-species reactive applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DsRed antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. DsRed protein, a red fluorescent protein derived from Discosoma coral, is notable for exceptional stability under extreme pH conditions and resistance to photobleaching, making DsRed an invaluable tool in fluorescence microscopy and live-cell imaging. One of the key features of DsRed is post-translational modifications, particularly the maturation process that leads to a significant red-shift in fluorescence from 558 nm to 583 nm upon maturation. This maturation not only enhances brightness but also allows for effective use of DsRed in dual-reporter systems alongside green fluorescent proteins, enabling researchers to visualize and differentiate between multiple proteins in complex biological systems. The ability to utilize DsRed in various expression systems, including bacterial, insect, and mammalian hosts, further underscores DsRed′s importance in molecular biology and biochemistry, facilitating the study of protein interactions and cellular dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DsRed Antibody (E-8) References:

1. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.  |  Baird, GS., et al. 2000. Proc Natl Acad Sci U S A. 97: 11984-9. PMID: 11050229
2. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed.  |  Wall, MA., et al. 2000. Nat Struct Biol. 7: 1133-8. PMID: 11101896
3. Red fluorescent protein (DsRed) as a reporter in Saccharomyces cerevisiae.  |  Rodrigues, F., et al. 2001. J Bacteriol. 183: 3791-4. PMID: 11371544
4. Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.  |  Zapata-Hommer, O. and Griesbeck, O. 2003. BMC Biotechnol. 3: 5. PMID: 12769828
5. Crystallographic structures of Discosoma red fluorescent protein with immature and mature chromophores: linking peptide bond trans-cis isomerization and acylimine formation in chromophore maturation.  |  Tubbs, JL., et al. 2005. Biochemistry. 44: 9833-40. PMID: 16026155
6. Noncytotoxic DsRed derivatives for whole-cell labeling.  |  Strack, RL., et al. 2011. Methods Mol Biol. 699: 355-70. PMID: 21116992
7. Sensitive DsRed fluorescence-based reporter cell systems for genotoxicity and oxidative stress assessment.  |  Hendriks, G., et al. 2011. Mutat Res. 709-710: 49-59. PMID: 21382384
8. Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis.  |  Carroll, P., et al. 2018. BMC Res Notes. 11: 685. PMID: 30285840
9. Expression of GFP and DsRed fluorescent proteins after gene electrotransfer of tumour cells in vitro.  |  Komel, T., et al. 2023. Bioelectrochemistry. 153: 108490. PMID: 37356264
10. Simultaneous EYFP-CENH3/H2B-DsRed Expression Is Impaired Differentially in Meristematic and Differentiated Nuclei of Arabidopsis Double Transformants.  |  Schubert, V., et al. 2023. Cytogenet Genome Res. 163: 74-80. PMID: 37552957
11. Data of crystal structure of the monomeric red fluorescent protein DsRed.  |  Nam, KH. 2024. Data Brief. 57: 110905. PMID: 39376484
12. Enhancer trapping with a red fluorescent protein reporter in Drosophila.  |  Akimoto, A., et al. 2005. Dev Dyn. 233: 993-7. PMID: 15906373

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DsRed Antibody (E-8) has 44 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DsRed Antibody (E-8) Specifications

Alternate Product Name: DsRed

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 178-223

Epitope Target: DsRed

Epitope Species: Discosoma Sp.

Product Reactivity/Detection: proteins containing the DsRed tag

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 44 citations in various scientific publications

Available Conjugates/Forms for DsRed Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin