DRP1 Antibody (C-5): sc-271583

DRP1 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DRP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DRP1 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dynamin-related protein 1 (DRP1), also known as Dynamin-like protein 1, Dlp1, DVLP, or Dymple, plays a crucial role in mediating outer mitochondrial membrane fission in mammalian cells, a process essential for mitochondrial dynamics and cellular health. DRP1 is characterized by an N-terminal tripartite GTP-binding domain, which is a hallmark of the Dynamin superfamily of GTPases. Structurally, DRP1 exists as a T-shaped dimer composed of a head, leg, and stalk, and GTP binding induces a conformational change that generates the mechanical force necessary for membrane constriction. DRP1 is ubiquitously expressed, with particularly high levels in skeletal muscle, heart, kidney, and brain tissues, and localizes to the perinuclear region within cells. In mouse brain, DRP1 shows high expression in the cerebellum, especially in Purkinje cells, highlighting DRP1′s importance in neuronal function. During apoptosis, DRP1 translocates from the cytosol to mitochondria, localizing to sites of organelle division, and DRP1 inhibition prevents cell death, underscoring the critical role of mitochondrial fission in the apoptotic process.

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DRP1 Antibody (C-5) References:

1. Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells.  |  Smirnova, E., et al. 2001. Mol Biol Cell. 12: 2245-56. PMID: 11514614
2. Three-dimensional reconstruction of dynamin in the constricted state.  |  Zhang, P. and Hinshaw, JE. 2001. Nat Cell Biol. 3: 922-6. PMID: 11584275
3. The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis.  |  Frank, S., et al. 2001. Dev Cell. 1: 515-25. PMID: 11703942
4. Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology.  |  Misaka, T., et al. 2002. J Biol Chem. 277: 15834-42. PMID: 11847212
5. Regulation of mitochondrial morphology by membrane potential, and DRP1-dependent division and FZO1-dependent fusion reaction in mammalian cells.  |  Ishihara, N., et al. 2003. Biochem Biophys Res Commun. 301: 891-8. PMID: 12589796
6. Intra- and intermolecular domain interactions of the C-terminal GTPase effector domain of the multimeric dynamin-like GTPase Drp1.  |  Zhu, PP., et al. 2004. J Biol Chem. 279: 35967-74. PMID: 15208300
7. Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis.  |  Lee, YJ., et al. 2004. Mol Biol Cell. 15: 5001-11. PMID: 15356267
8. Drp1/Fis1 interaction mediates mitochondrial dysfunction in septic cardiomyopathy.  |  Haileselassie, B., et al. 2019. J Mol Cell Cardiol. 130: 160-169. PMID: 30981733
9. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.  |  Zhou, CJ., et al. 2022. Nat Commun. 13: 7732. PMID: 36513638
10. Limiting mitochondrial plasticity by targeting DRP1 induces metabolic reprogramming and reduces breast cancer brain metastases.  |  Parida, PK., et al. 2023. Nat Cancer. 4: 893-907. PMID: 37248394
11. Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p.  |  Shin, HW., et al. 1997. J Biochem. 122: 525-30. PMID: 9348079
12. Identification and functional characterization of a novel human protein highly related to the yeast dynamin-like GTPase Vps1p.  |  Imoto, M., et al. 1998. J Cell Sci. 111 (Pt 10): 1341-9. PMID: 9570752

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DRP1 Antibody (C-5) has 149 citations in a variety of scientific publications.

Primary antibodies like Anti-DRP1 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DRP1 Antibody (C-5) Specifications

Alternate Product Name: DRP1

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 560-736

Epitope Target: DRP1

Epitope Species: human

Product Reactivity/Detection: all DRP1 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 149 citations in various scientific publications

Available Conjugates/Forms for DRP1 Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred DRP1 antibody is DRP1 Antibody (6Z-82): sc-101270.