DRAM Antibody (M3-P4B4): sc-81713
- DRAM Antibody (M3-P4B4) is a mouse monoclonal IgG1 λ DRAM antibody, cited in 8 publications, provided at 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids 192-201 of DRAM of human origin
- DRAM Antibody (M3-P4B4) is recommended for detection of DRAM of human origin by WB, IP and IF
- Anti-DRAM Antibody (M3-P4B4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DRAM Antibody (M3-P4B4) for WB applications. These reagents are now offered in bundles with DRAM Antibody (M3-P4B4) (see ordering information below).
QUICK LINKS
SEE ALSO...
DRAM Antibody (M3-P4B4) is a mouse monoclonal IgG1 antibody that detects DRAM protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). DRAM (M3-P4B4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DRAM protein, or damage-regulated autophagy modulator, is a multi-pass membrane protein belonging to the TMEM77 family and is primarily localized to the lysosomal membrane. This localization is crucial as DRAM plays a significant role in lysosomal function, particularly in the degradation of cellular components and the regulation of autophagy. DRAM is highly conserved across various species and features six transmembrane domains along with an endoplasmic reticulum signal peptide, which are essential for proper functioning and integration into the lysosomal membrane. DRAM expression is notably induced by tumor suppressor proteins p53 and p73, positioning DRAM as a vital component in p53-mediated autophagy and apoptosis, although DRAM is not required for p73-mediated apoptosis. Furthermore, downregulation of DRAM expression in human cancers highlights DRAM′s potential role in tumorigenesis, making DRAM an important target for research in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DRAM Antibody (M3-P4B4) References:
- p53 and metabolism: Inside the TIGAR. | Green, DR. and Chipuk, JE. 2006. Cell. 126: 30-2. PMID: 16839873
- DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. | Crighton, D., et al. 2006. Cell. 126: 121-34. PMID: 16839881
- DRAM links autophagy to p53 and programmed cell death. | Crighton, D., et al. 2007. Autophagy. 3: 72-4. PMID: 17102582
- p73 regulates DRAM-independent autophagy that does not contribute to programmed cell death. | Crighton, D., et al. 2007. Cell Death Differ. 14: 1071-9. PMID: 17304243
- The direct p53 target gene, FLJ11259/DRAM, is a member of a novel family of transmembrane proteins. | Kerley-Hamilton, JS., et al. 2007. Biochim Biophys Acta. 1769: 209-19. PMID: 17397945
- p53-Dependent and p53-independent activation of autophagy by ARF. | Abida, WM. and Gu, W. 2008. Cancer Res. 68: 352-7. PMID: 18199527
- Analysis of DRAM-related proteins reveals evolutionarily conserved and divergent roles in the control of autophagy. | O'Prey, J., et al. 2009. Cell Cycle. 8: 2260-5. PMID: 19556885
- DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose. | Mrschtik, M., et al. 2015. Cell Death Differ. 22: 1714-26. PMID: 25929859
- Relationship between diastasis of the rectus abdominis muscle (DRAM) and musculoskeletal dysfunctions, pain and quality of life: a systematic review. | Benjamin, DR., et al. 2019. Physiotherapy. 105: 24-34. PMID: 30217494
- DRAM for distilling microbial metabolism to automate the curation of microbiome function. | Shaffer, M., et al. 2020. Nucleic Acids Res. 48: 8883-8900. PMID: 32766782
- DRAM-4 and DRAM-5 are compensatory regulators of autophagy and cell survival in nutrient-deprived conditions. | Barthet, VJA., et al. 2022. FEBS J. 289: 3752-3769. PMID: 35060334
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DRAM Antibody (M3-P4B4) | sc-81713 | 200 µg/ml | $322.00 | |||
DRAM Antibody (M3-P4B4): m-IgG Fc BP-HRP Bundle | sc-527062 | 200 µg Ab; 10 µg BP | $361.00 | |||
DRAM Antibody (M3-P4B4): m-IgG1 BP-HRP Bundle | sc-532435 | 200 µg Ab; 20 µg BP | $361.00 | |||
DRAM Antibody (M3-P4B4) AC | sc-81713 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DRAM Antibody (M3-P4B4) HRP | sc-81713 HRP | 200 µg/ml | $322.00 | |||
DRAM Antibody (M3-P4B4) FITC | sc-81713 FITC | 200 µg/ml | $336.00 | |||
DRAM Antibody (M3-P4B4) PE | sc-81713 PE | 200 µg/ml | $349.00 | |||
DRAM Antibody (M3-P4B4) Alexa Fluor® 488 | sc-81713 AF488 | 200 µg/ml | $364.00 | |||
DRAM Antibody (M3-P4B4) Alexa Fluor® 546 | sc-81713 AF546 | 200 µg/ml | $364.00 | |||
DRAM Antibody (M3-P4B4) Alexa Fluor® 594 | sc-81713 AF594 | 200 µg/ml | $364.00 | |||
DRAM Antibody (M3-P4B4) Alexa Fluor® 647 | sc-81713 AF647 | 200 µg/ml | $364.00 | |||
DRAM Antibody (M3-P4B4) Alexa Fluor® 680 | sc-81713 AF680 | 200 µg/ml | $364.00 | |||
DRAM Antibody (M3-P4B4) Alexa Fluor® 790 | sc-81713 AF790 | 200 µg/ml | $364.00 |