Date published: 2026-5-4
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DR5 Antibody (D-6) - Immunofluorescence - Image 145442
DR5 Antibody (D-6): sc-166624. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
DR5 Antibody (D-6): sc-166624. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

DR5 Antibody (D-6): sc-166624

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Datasheets
  • DR5 Antibody (D-6) is a mouse monoclonal IgG1 κ DR5 antibody, cited in 38 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 48-76 within an internal region of DR5 of human origin
  • DR5 Antibody (D-6) is recommended for detection of DR5 of human origin by WB, IP, IF and ELISA
  • Anti-DR5 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DR5 Antibody (D-6) for WB applications. These reagents are now offered in bundles with DR5 Antibody (D-6) (see ordering information below).
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Additional DR Antibodies including DR3, DR4 and DR6

DR5 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects DR5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DR5 antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DR5 (TNFRSF10B) plays a crucial role in mediating apoptosis through interaction with cytokine TRAIL (TNF-related apoptosis-inducing ligand). This interaction regulates programmed cell death, essential for maintaining cellular homeostasis and eliminating damaged or cancerous cells. DR5′s ability to initiate apoptosis makes DR5 a significant target in cancer therapy, as enhancing DR5 signaling pathway can promote death of tumor cells while sparing normal cells. DR5 belongs to tumor necrosis factor receptor superfamily, which includes receptors like FAS and TNF-R1, underscoring DR5′s relevance in immune responses and therapeutic interventions.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

DR5 Antibody (D-6) References:

  1. Targeting of keloid with TRAIL and TRAIL-R2/DR5.  |  Sun, P., et al. 2021. J Dermatolog Treat. 32: 957-964. PMID: 31916474
  2. Restoring TRAILR2/DR5-Mediated Activation of Apoptosis upon Endoplasmic Reticulum Stress as a Therapeutic Strategy in Cancer.  |  Mora-Molina, R. and López-Rivas, A. 2022. Int J Mol Sci. 23: PMID: 36012252
  3. DR5-Selective TRAIL Variant DR5-B Functionalized with Tumor-Penetrating iRGD Peptide for Enhanced Antitumor Activity against Glioblastoma.  |  Yagolovich, AV., et al. 2022. Int J Mol Sci. 23: PMID: 36293545
  4. DR5 disulfide bonding as a sensor and effector of protein folding stress.  |  Law, ME., et al. 2024. bioRxiv.. PMID: 38496520
  5. The Fas death factor.  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
  6. The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death.  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
  7. A novel domain within the 55 kd TNF receptor signals cell death.  |  Tartaglia, LA., et al. 1993. Cell. 74: 845-53. PMID: 8397073
  8. A death-domain-containing receptor that mediates apoptosis.  |  Kitson, J., et al. 1996. Nature. 384: 372-5. PMID: 8934525
  9. The receptor for the cytotoxic ligand TRAIL.  |  Pan, G., et al. 1997. Science. 276: 111-3. PMID: 9082980
  10. An antagonist decoy receptor and a death domain-containing receptor for TRAIL.  |  Pan, G., et al. 1997. Science. 277: 815-8. PMID: 9242610
  11. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.  |  Sheridan, JP., et al. 1997. Science. 277: 818-21. PMID: 9242611
  12. TRUNDD, a new member of the TRAIL receptor family that antagonizes TRAIL signalling.  |  Pan, G., et al. 1998. FEBS Lett. 424: 41-5. PMID: 9537512

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DR5 Antibody (D-6)

sc-166624
200 µg/ml
$322.00

DR5 Antibody (D-6): m-IgG Fc BP-HRP Bundle

sc-529008
200 µg Ab; 10 µg BP
$361.00

DR5 Antibody (D-6): m-IgGκ BP-HRP Bundle

sc-521679
200 µg Ab, 40 µg BP
$361.00

DR5 Antibody (D-6): m-IgG1 BP-HRP Bundle

sc-543265
200 µg Ab; 20 µg BP
$361.00

DR5 Antibody (D-6) AC

sc-166624 AC
500 µg/ml, 25% agarose
$424.00

DR5 Antibody (D-6) HRP

sc-166624 HRP
200 µg/ml
$322.00

DR5 Antibody (D-6) FITC

sc-166624 FITC
200 µg/ml
$336.00

DR5 Antibody (D-6) PE

sc-166624 PE
200 µg/ml
$349.00

DR5 Antibody (D-6) Alexa Fluor® 488

sc-166624 AF488
200 µg/ml
$364.00

DR5 Antibody (D-6) Alexa Fluor® 546

sc-166624 AF546
200 µg/ml
$364.00

DR5 Antibody (D-6) Alexa Fluor® 594

sc-166624 AF594
200 µg/ml
$364.00

DR5 Antibody (D-6) Alexa Fluor® 647

sc-166624 AF647
200 µg/ml
$364.00

DR5 Antibody (D-6) Alexa Fluor® 680

sc-166624 AF680
200 µg/ml
$364.00

DR5 Antibody (D-6) Alexa Fluor® 790

sc-166624 AF790
200 µg/ml
$364.00

DR5 (D-6) Neutralizing Peptide

sc-166624 P
100 µg/0.5 ml
$69.00

I want to use DR5 (D-6): sc-166624 monoclonal antibody for Western blot. What is the recommended starting dilution for this antibody?

Asked by: cjMara
We recommend using DR5 (D-6): sc-166624 mouse monoclonal antibody at a starting dilution of 1:200 for Western blot (range of 1:100-1:1,000).
Answered by: Technical Support 15
Date published: 2017-01-20

Is it antibody targeting the internal or external part of the receptor? According to RCSB the epitope mapping between amino acids 48-76 falls in the extracellular part of the receptor?

Asked by: YHua
Thank you for your question. This antibody is specific for an epitope mapping between amino acids 48-76 within an internal region of DR5 of human origin and should detect the extracellular part of the DR5 (amino acids 56-444 are extracellular per accession#O14763). Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2016-10-24
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Rated 5 out of 5 by from 不错新用户，期待收货。希望合作愉快！售后、售前工作希望能洽谈愉快！之前在其他平台订货，第一次在这下单，期待
Date published: 2022-06-27
Rated 4 out of 5 by from Clear and good signalPrimary antibody DR5 (D-6) sc-166624 was used diluted 1:500 according to the manufacturer’s recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membranes of PVDF have been incubated overnight at 4°C and then they have been probed for 1h at RT with secondary antibodies diluted 1:5000 in Milk 2,5%. We have tested this antibodiy on melanoma cell line A375 and A2058. In both cases we obtained a clear and quite good signal, so we can conclude that the antibody has worked very well.
Date published: 2020-11-09
Rated 1 out of 5 by from tested on rat cells (NRK52E)Tested in WB on NRK52E cell lysate + BJAB cell lysate (used as positive control) Expected MW at 48kDa We have observed 2 different bands in WB at 72kDa and 25kDa. Nothing at 48kda. Probably, it is possible that the antibody is not specific for rat cells. In any case, we have seen similar results also in BJAB cell lysate (no clear band at 48kDa).
Date published: 2020-08-10
Rated 1 out of 5 by from does not work for western in MDA-MB-231 cellsThis DR-5 antibody does not work for western in MDA-MB-231 cells
Date published: 2018-02-02
Rated 5 out of 5 by from Good antibody!I bought this antibody for Western blot experiments and the specific bands could be observed by using this antibody!
Date published: 2017-08-02
Rated 5 out of 5 by from GOOD!I got this antibody sample two weeks ago, and I am very glad that this antibody is not bad. I uesd DR5 of 1:100 dilution in WB and second antibody is 1:2000 dilution, loaded 60 ug.
Date published: 2017-05-31
Rated 5 out of 5 by from Good antibodyThe clean band was got with this antibody in western blot.
Date published: 2017-05-22
Rated 5 out of 5 by from Good WB resultsUsed it for WB experiment and worked perfectly. Good quality.
Date published: 2017-03-29
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DR5 Antibody (D-6) is rated 4.4 out of 5 by 14.
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