DP-2 Antibody (D-11): sc-374614
- DP-2 Antibody (D-11) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-31 at the N-terminus of DP-2 of human origin
- Anti-DP-2 Antibody (D-11) is recommended for detection of DP-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, bovine and porcine
- TransCruz reagent for ChIP application (sc-374614 X, 200 µg/0.1 ml)
- Anti-DP-2 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DP-2 Antibody (D-11) for WB applications. These reagents are now offered in bundles with DP-2 Antibody (D-11) (see ordering information below).
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DP-2 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects DP-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DP-2 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DP-2 plays a crucial role in cell cycle regulation, particularly in the transition from G1 phase to S phase, by interacting with E2F transcription factor family. This interaction ensures cells only progress through the cycle when conditions are favorable, making proper control of cell proliferation possible. Dysregulation of this process can lead to uncontrolled cell growth and is often implicated in cancer development. DP-2′s ability to form heterodimers with E2F-1, creating an active transcriptional regulatory complex, highlights its importance in cellular function and potential consequences of malfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DP-2 Antibody (D-11) References:
- E2F: a link between the Rb tumor suppressor protein and viral oncoproteins. | Nevins, JR. 1992. Science. 258: 424-9. PMID: 1411535
- Identification and characterization of novel isoforms of human DP-1: DP-1{alpha} regulates the transcriptional activity of E2F1 as well as cell cycle progression in a dominant-negative manner. | Ishida, H., et al. 2005. J Biol Chem. 280: 24642-8. PMID: 15863509
- A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F. | Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
- Heterogeneous cross-talk of E2F family members is crucially involved in growth modulatory effects of interferon-gamma and EGF. | Reimer, D., et al. 2006. Cancer Biol Ther. 5: 771-6. PMID: 16721044
- Cloning and characterization of human DP2, a novel dimerization partner of E2F. | Zhang, Y. and Chellappan, SP. 1995. Oncogene. 10: 2085-93. PMID: 7784053
- E2F-4, a new member of the E2F transcription factor family, interacts with p107. | Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
- E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo. | Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
- Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members. | Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
- Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. | Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DP-2 Antibody (D-11) | sc-374614 | 200 µg/ml | $322.00 | |||
DP-2 Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-529782 | 200 µg Ab; 10 µg BP | $361.00 | |||
DP-2 Antibody (D-11): m-IgGκ BP-HRP Bundle | sc-522792 | 200 µg Ab, 40 µg BP | $361.00 | |||
DP-2 Antibody (D-11): m-IgG1 BP-HRP Bundle | sc-543688 | 200 µg Ab; 20 µg BP | $361.00 | |||
DP-2 Antibody (D-11) X | sc-374614 X | 200 µg/0.1 ml | $322.00 | |||
DP-2 Antibody (D-11) AC | sc-374614 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DP-2 Antibody (D-11) HRP | sc-374614 HRP | 200 µg/ml | $322.00 | |||
DP-2 Antibody (D-11) FITC | sc-374614 FITC | 200 µg/ml | $336.00 | |||
DP-2 Antibody (D-11) PE | sc-374614 PE | 200 µg/ml | $349.00 | |||
DP-2 Antibody (D-11) Alexa Fluor® 488 | sc-374614 AF488 | 200 µg/ml | $364.00 | |||
DP-2 Antibody (D-11) Alexa Fluor® 546 | sc-374614 AF546 | 200 µg/ml | $364.00 | |||
DP-2 Antibody (D-11) Alexa Fluor® 594 | sc-374614 AF594 | 200 µg/ml | $364.00 | |||
DP-2 Antibody (D-11) Alexa Fluor® 647 | sc-374614 AF647 | 200 µg/ml | $364.00 | |||
DP-2 Antibody (D-11) Alexa Fluor® 680 | sc-374614 AF680 | 200 µg/ml | $364.00 | |||
DP-2 Antibody (D-11) Alexa Fluor® 790 | sc-374614 AF790 | 200 µg/ml | $364.00 | |||
DP-2 (D-11) Neutralizing Peptide | sc-374614 P | 100 µg/0.5 ml | $69.00 |