Date published: 2025-9-5
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Doublecortin/DCX Antibody (E-5) - Western Blotting - Image 379655
双皮质素抗体 (E-5): sc-390645. IMR-32 (A), C2C12 (B) 和 U-251-MG (C) 全细胞裂解液中双皮质素表达的 Western 印迹分析.
Doublecortin/DCX Antibody (E-5) - Western Blotting - Image 379655
双皮质素抗体 (E-5): sc-390645. IMR-32 (A), C2C12 (B) 和 U-251-MG (C) 全细胞裂解液中双皮质素表达的 Western 印迹分析.
Doublecortin/DCX Antibody (E-5) - Immunohistochemistry - Image 348271
双皮质素抗体 (E-5): sc-390645. 对福尔马林固定, 石蜡包埋的人大脑皮层组织进行免疫过氧化物酶染色, 显示神经元细胞和胶质细胞的胞浆染色.
Doublecortin/DCX Antibody (E-5) - Western Blotting - Image 289678
双皮质素抗体 (E-5): sc-390645. 未转染 293T: sc-117752 (A), 转染人 Doublecortin 的 293T: sc-114231 (B) 和 IMR-32 (C) 全细胞裂解液中 Doublecortin 表达的 Western 印迹分析.
双皮质素抗体 (E-5): sc-390645. IMR-32 (A), C2C12 (B) 和 U-251-MG (C) 全细胞裂解液中双皮质素表达的 Western 印迹分析.

Doublecortin/DCX 抗体 (E-5): sc-390645

4.2(6)
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说明书
  • Doublecortin/DCX抗体(E-5)是小鼠单克隆IgG1 (kappa轻链)，规格为200 µg/ml
  • 与human的46-79氨基酸残基的Doublecortin
  • 抗-Doublecortin/DCX 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Doublecortin; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Doublecortin/DCX抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Doublecortin/DCX (E-5): sc-390645的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Doublecortin/DCX 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Doublecortin/DCX 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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    相关产品
    描述
    基因信息
    说明书与实验方案
    研究信息

    関連項目

    Doublecortin抗体（E-5）是一种IgG1 κ小鼠单克隆Doublecortin抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的Doublecortin。Doublecortin抗体（E-5）既可以是非偶联的抗Doublecortin抗体形式，也可以是多种偶联形式的抗Doublecortin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。无脑回畸形（平滑脑）是一种大脑发育异常，其特征是神经元迁移不完全和大脑表面光滑，导致严重的智力障碍。遗传分析确定了在一些无脑回畸形病例中发生突变的两种蛋白质，称为无脑回畸形-1蛋白（LIS1）和doublecortin。LIS1与异源三聚体G蛋白的β亚基具有序列同源性。Doublecortin包含一个共识的Abl磷酸化位点，并且与预测的激酶蛋白具有一些序列同源性。这两种蛋白质在发育中的大脑中高度表达，这表明它们可能参与对大脑发育至关重要的信号转导途径。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Doublecortin/DCX 抗体 (E-5) 参考文献:

    1. 双皮质蛋白，一种微管的稳定剂。  |  Horesh, D., et al. 1999. Hum Mol Genet. 8: 1599-610. PMID: 10441322
    2. 在发育中的神经系统中，双皮质蛋白与微囊蛋白复合物的μ亚基相互作用。  |  Friocourt, G., et al. 2001. Mol Cell Neurosci. 18: 307-19. PMID: 11591131
    3. 双皮质蛋白和双皮质蛋白样激酶的DCX-domain串联。  |  Kim, MH., et al. 2003. Nat Struct Biol. 10: 324-33. PMID: 12692530
    4. 双皮质蛋白在生长中的神经突起的末端发挥作用。  |  Friocourt, G., et al. 2003. Cereb Cortex. 13: 620-6. PMID: 12764037
    5. 双皮质蛋白的DC模块：动力学、结构域边界和功能影响。  |  Cierpicki, T., et al. 2006. Proteins. 64: 874-82. PMID: 16835924
    6. 双皮质素基因家族与神经元结构紊乱。  |  Dijkmans, TF., et al. 2010. Cent Nerv Syst Agents Med Chem. 10: 32-46. PMID: 20236041
    7. 人类双皮质蛋白C端泛素样结构域的域互换。  |  Rufer, AC., et al. 2018. Acta Crystallogr D Struct Biol. 74: 450-462. PMID: 29717716
    8. 缺乏 MeCP2 会导致嗅觉系统中双皮质素的特异性区域性增加。  |  Martínez-Rodríguez, E., et al. 2019. Brain Struct Funct. 224: 1647-1658. PMID: 30923887
    9. KIAA0369的表达和染色体定位，该蛋白是一种与双皮质蛋白结构相关的假定激酶。  |  Omori, Y., et al. 1998. J Hum Genet. 43: 169-77. PMID: 9747029
    10. 双皮质蛋白小鼠同源物的克隆和发育表达。  |  Matsuo, N., et al. 1998. Biochem Biophys Res Commun. 252: 571-6. PMID: 9837748

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Doublecortin/DCX 抗体 (E-5)

    sc-390645
    200 µg/ml
    		$316.00

    Doublecortin/DCX (E-5): m-IgG Fc BP-HRP 套装

    sc-530212
    200 µg Ab; 10 µg BP
    		$354.00

    Doublecortin/DCX (E-5): m-IgGκ BP-HRP 套装

    sc-523616
    200 µg Ab, 40 µg BP
    		$354.00

    Doublecortin/DCX 抗体 (E-5) AC

    sc-390645 AC
    500 µg/ml, 25% agarose
    		$416.00

    Doublecortin/DCX 抗体 (E-5) HRP

    sc-390645 HRP
    200 µg/ml
    		$316.00

    Doublecortin/DCX 抗体 (E-5) FITC

    sc-390645 FITC
    200 µg/ml
    		$330.00

    Doublecortin/DCX 抗体 (E-5) PE

    sc-390645 PE
    200 µg/ml
    		$343.00

    Doublecortin/DCX 抗体 (E-5) Alexa Fluor® 488

    sc-390645 AF488
    200 µg/ml
    		$357.00

    Doublecortin/DCX 抗体 (E-5) Alexa Fluor® 546

    sc-390645 AF546
    200 µg/ml
    		$357.00

    Doublecortin/DCX 抗体 (E-5) Alexa Fluor® 594

    sc-390645 AF594
    200 µg/ml
    		$357.00

    Doublecortin/DCX 抗体 (E-5) Alexa Fluor® 647

    sc-390645 AF647
    200 µg/ml
    		$357.00

    Doublecortin/DCX 抗体 (E-5) Alexa Fluor® 680

    sc-390645 AF680
    200 µg/ml
    		$357.00

    Doublecortin/DCX 抗体 (E-5) Alexa Fluor® 790

    sc-390645 AF790
    200 µg/ml
    		$357.00

    Doublecortin/DCX (E-5) 中和勝肽

    sc-390645 P
    100 µg/0.5 ml
    		$68.00

    What is the difference between E-5 and E-6 Doublecortin antibodies?

    Asked by: Sil38
    Thank you for your question. These are two difference mouse monoclonal antibody clones that will detect the protein Doublecortin. They are made against different epitopes, so they detect different parts of the Doublecortin protein. Please reach out to Technical service of your local distributor if you would like further assistance choosing the optimal antibody for your research. We're happy to help!
    Answered by: Tech Service
    Date published: 2020-02-04

    Dear Sir/ Madam I have some quetion. Can I use Doublecortin Antibody (E-5): sc-390645 for 4-6weeks rat pups and adult rats. Can you reccomend me suistable secondary antibody and kit for Doublecortin Antibody (E-5): sc-390645. Best regarts Nino

    Asked by: dodo37
    Thank you for your question. Our mouse monoclonal antibody reagents are not recommended or warrantied for in vivo applications, and we can not provide recommendations for that use. You can find our recommended secondary reagents here: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
    Answered by: Tech Service
    Date published: 2020-01-30

    Does sc390645 require antigen retrieval for frozen sections

    Asked by: ASWATHY
    Thank you for your question. We have not specifically tested this mouse monoclonal, Doublecortin Antibody (E-5): sc-390645, on PFA-fixed frozen tissue sections. However, based on our experience with other antibodies, it may not be necessary to include antigen retrieval in this case. If signal is not as strong as desired, then we do recommend performing antigen retrieval with a 1% SDS treatment for 5 minutes to enhance antibody signal on PFA-fixed frozen tissues.
    Answered by: Technical Support
    Date published: 2018-12-04

    What is the recommended secondary reagent to be used with this mouse monoclonal primary?

    Asked by: jerojero
    We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
    Answered by: Technical Support 14
    Date published: 2017-02-07
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    Rated 5 out of 5 by from great deliveryAll items received with Sales Order No 6333110 are with great quality!
    Date published: 2022-08-17
    Rated 5 out of 5 by from Embrio cultureThe antibody is used to identify progenitor cells of neurons in culture of embryos treated with valproic acid?
    Date published: 2018-09-03
    Rated 1 out of 5 by from Feedback acerca do anticorpo DCX (E-5): sc-390645Pedi uma aliquota de teste do anticorpo DCX (E-5) sc-390645 e fiz uma imunocitoquímica em tecido de ratinho e rato. Para ambos os casos, o anticorpo não funcionou. De qualquer das formas, obrigada por terem fornecido a alíquota de teste.
    Date published: 2017-12-21
    Rated 4 out of 5 by from Nice StainingTried a sample out for ICC. The results were very nice.
    Date published: 2017-11-02
    Rated 5 out of 5 by from Preferred replacement for (N-19)The (E-5) clone is the preferred replacement for goat polyclonal IgG (N-19). This clone is raised against the same peptide, and works well in IHC(P), IF and Western blotting applications, without any lot-to-lot variation.
    Date published: 2017-01-23
    Rated 5 out of 5 by from Monoclonal antibody with excellent resultsMonoclonal antibody with excellent results in Western blot. Endogenous detection in IMR-32 whole cell lysate and confirmation of specificity with a human transfected cell lysate.
    Date published: 2014-06-12
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    Doublecortin/DCX 抗体 (E-5) is rated 4.2 out of 5 by 6.
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