Dok-3 Antibody (E-2): sc-271132
- Dok-3 Antibody (E-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 231-444 mapping at the C-terminus of Dok-3 of mouse origin
- recommended for detection of Dok-3 of mouse origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dok-3 Antibody (E-2) for WB applications. These reagents are now offered in bundles with Dok-3 Antibody (E-2) (see ordering information below).
Dok-3 Antibody (E-2) is a mouse monoclonal IgG1 antibody that detects Dok-3 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dok-3 is a crucial adapter protein that plays a significant role in the recruitment of inhibitory molecules, particularly in immune cells like B cells and macrophages. Dok-3 expression is notably elevated in these cell types, where Dok-3 becomes activated upon immunoreceptor-mediated cellular stimulation, leading to tyrosine phosphorylation. This post-translational modification enables Dok-3 to interact with key signaling proteins, including the 5′ inositol phosphatase SHIP and the protein tyrosine kinase Csk, thereby contributing to the negative regulation of immunoreceptor signaling pathways. Dok-3′s ability to modulate these pathways is essential for maintaining immune homeostasis and preventing overactivation of immune responses, which can lead to autoimmune disorders. Anti-Dok-3 antibody (E-2) is an invaluable tool for researchers studying immune signaling and the intricate mechanisms of cellular regulation.
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Dok-3 Antibody (E-2) References:
- Dok-3 sequesters Grb2 and inhibits the Ras-Erk pathway downstream of protein-tyrosine kinases. | Honma, M., et al. 2006. Genes Cells. 11: 143-51. PMID: 16436051
- Dok-3 plays a nonredundant role in negative regulation of B-cell activation. | Ng, CH., et al. 2007. Blood. 110: 259-66. PMID: 17363732
- DOK3 is required for IFN-β production by enabling TRAF3/TBK1 complex formation and IRF3 activation. | Kim, SS., et al. 2014. J Immunol. 193: 840-8. PMID: 24929003
- Adaptor protein DOK3 promotes plasma cell differentiation by regulating the expression of programmed cell death 1 ligands. | Ou, X., et al. 2014. Proc Natl Acad Sci U S A. 111: 11431-6. PMID: 25053811
- TRAF6-mediated degradation of DOK3 is required for production of IL-6 and TNFα in TLR9 signaling. | Liu, N., et al. 2015. Mol Immunol. 68: 699-705. PMID: 26548852
- DOK3 Degradation is Required for the Development of LPS-induced ARDS in Mice. | Liu, N., et al. 2016. Curr Gene Ther. 16: 256-262. PMID: 28590889
- DOK3 Modulates Bone Remodeling by Negatively Regulating Osteoclastogenesis and Positively Regulating Osteoblastogenesis. | Cai, X., et al. 2017. J Bone Miner Res. 32: 2207-2218. PMID: 28650106
- ULK1 Suppresses Osteoclast Differentiation and Bone Resorption via Inhibiting Syk-JNK through DOK3. | Zhang, Y., et al. 2021. Oxid Med Cell Longev. 2021: 2896674. PMID: 34820053
- Plasma exosomal DOK3 reflects immunological states in lung tumor and predicts prognosis of gefitinib treatment. | Ochiai, R., et al. 2022. Cancer Sci. 113: 3960-3971. PMID: 36028467
- DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11. | Loh, JT., et al. 2023. Sci Signal. 16: eadg5171. PMID: 37906628
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dok-3 Antibody (E-2) | sc-271132 | 200 µg/ml | $322.00 | |||
Dok-3 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-537571 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dok-3 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-534874 | 200 µg Ab; 40 µg BP | $361.00 | |||
Dok-3 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-545236 | 200 µg Ab; 20 µg BP | $361.00 |