Dok-3 Antibody (F-7): sc-390007

Dok-3 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dok-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Dok-3 antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dok-3 is a crucial adapter involved in the recruitment of inhibitory molecules, particularly in immune cells such as B cells and macrophages, where Dok-3 plays a significant role in regulating immunoreceptor signaling. Upon immunoreceptor-mediated activation, Dok-3 undergoes tyrosine phosphorylation, which facilitates interaction with key signaling proteins, including the 5′ inositol phosphatase SHIP and the protein tyrosine kinase Csk. This interaction is vital for the negative regulation of signaling pathways that can lead to excessive immune responses, highlighting Dok-3′s importance in maintaining immune homeostasis. Additionally, Dok-3 is part of a family of docking proteins that contain multiple tyrosine residues and potential SH2 binding sites, which are essential for mediating various cellular signaling processes. Dok-3′s ability to modulate these pathways underscores its significance in both normal immune function and the potential development of immune-related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dok-3 Antibody (F-7) References:

1. Characterization of a novel member of the DOK family that binds and modulates Abl signaling.  |  Cong, F., et al. 1999. Mol Cell Biol. 19: 8314-25. PMID: 10567556
2. Dok3-protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity.  |  Loh, JT., et al. 2019. J Clin Invest. 129: 2717-2729. PMID: 31180338
3. DOK3 is involved in microglial cell activation in neuropathic pain by interacting with GPR84.  |  Gao, WS., et al. 2020. Aging (Albany NY). 13: 389-410. PMID: 33281117
4. Dok3 is involved in cisplatin-induced acute kidney injury via regulation of inflammation and apoptosis.  |  Yang, YY., et al. 2021. Biochem Biophys Res Commun. 569: 132-138. PMID: 34245977
5. DOK3 promotes proliferation and inhibits apoptosis of prostate cancer via the NF-κB signaling pathway.  |  Jin, K., et al. 2023. Chin Med J (Engl). 136: 423-432. PMID: 36867541
6. Loss of Dok-3 in Non-tumor Cells Induces Malignant Transformation of Benign Epithelial Tumor Cells of the Intestine.  |  Arimura, S., et al. 2022. Cancer Res Commun. 2: 1590-1600. PMID: 36970719
7. Expression and clinical significance of DOK3 in renal clear cell carcinoma.  |  Wang, YF., et al. 2023. J Int Med Res. 51: 3000605231174974. PMID: 37235715
8. Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases.  |  Mayer, BJ., et al. 1995. Curr Biol. 5: 296-305. PMID: 7780740
9. A 62-kilodalton tyrosine phosphoprotein constitutively present in primary chronic phase chronic myelogenous leukemia enriched lineage negative blast populations.  |  Wisniewski, D., et al. 1994. Leukemia. 8: 688-93. PMID: 8152267
10. p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells.  |  Carpino, N., et al. 1997. Cell. 88: 197-204. PMID: 9008160
11. Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok.  |  Yamanashi, Y. and Baltimore, D. 1997. Cell. 88: 205-11. PMID: 9008161
12. Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins.  |  Di Cristofano, A., et al. 1998. J Biol Chem. 273: 4827-30. PMID: 9478921

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dok-3 Antibody (F-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Dok-3 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dok-3 Antibody (F-7) Specifications

Alternate Product Name: Dok-3

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 291-496

Epitope Target: Dok-3

Epitope Species: human

Product Reactivity/Detection: Dok-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Dok-3 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin