Dok-2 Antibody (E-10): sc-17830

Dok-2 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dok-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Dok-2 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dok-2, also known as p56 Dok, plays a crucial role in cellular signaling pathways, particularly in growth factor receptor activation. Dok-2 is primarily located in the cytoplasm and regulates Ras signaling, which is essential for cell proliferation and differentiation. Dok-2 interaction with Ras GTPase activating proteins highlights Dok-2′s importance in modulating Ras activity, a key player in many signaling cascades that control cell growth and survival. Dysregulation of this pathway can lead to various diseases, including cancer, making Dok-2 and its interactions vital for understanding tumorigenesis and developing targeted therapies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dok-2 Antibody (E-10) References:

1. Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases.  |  Mayer, BJ., et al. 1995. Curr Biol. 5: 296-305. PMID: 7780740
2. Vascular endothelial cell growth factor promotes tyrosine phosphorylation of mediators of signal transduction that contain SH2 domains. Association with endothelial cell proliferation.  |  Guo, D., et al. 1995. J Biol Chem. 270: 6729-33. PMID: 7896817
3. The IRS-1 signaling system.  |  Myers, MG., et al. 1994. Trends Biochem Sci. 19: 289-93. PMID: 8048169
4. A 62-kilodalton tyrosine phosphoprotein constitutively present in primary chronic phase chronic myelogenous leukemia enriched lineage negative blast populations.  |  Wisniewski, D., et al. 1994. Leukemia. 8: 688-93. PMID: 8152267
5. A Grb2-associated docking protein in EGF- and insulin-receptor signalling.  |  Holgado-Madruga, M., et al. 1996. Nature. 379: 560-4. PMID: 8596638
6. p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells.  |  Carpino, N., et al. 1997. Cell. 88: 197-204. PMID: 9008160
7. Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok.  |  Yamanashi, Y. and Baltimore, D. 1997. Cell. 88: 205-11. PMID: 9008161
8. Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins.  |  Di Cristofano, A., et al. 1998. J Biol Chem. 273: 4827-30. PMID: 9478921

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dok-2 Antibody (E-10) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Dok-2 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dok-2 Antibody (E-10) Specifications

Alternate Product Name: Dok-2

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 221-412

Epitope Target: Dok-2

Epitope Species: human

Product Reactivity/Detection: Dok-2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Dok-2 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin