Dok-2 Antibody (E-10): sc-17830
- Dok-2 Antibody (E-10) is a mouse monoclonal IgG1 κ Dok-2 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 221-412 mapping at the C-terminus of Dok-2 of human origin
- Dok-2 Antibody (E-10) is recommended for detection of Dok-2 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Dok-2 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dok-2 Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Dok-2 Antibody (E-10) (see ordering information below).
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Dok-2 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dok-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Dok-2 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dok-2, also known as p56 Dok, plays a crucial role in cellular signaling pathways, particularly in growth factor receptor activation. Dok-2 is primarily located in the cytoplasm and regulates Ras signaling, which is essential for cell proliferation and differentiation. Dok-2 interaction with Ras GTPase activating proteins highlights Dok-2′s importance in modulating Ras activity, a key player in many signaling cascades that control cell growth and survival. Dysregulation of this pathway can lead to various diseases, including cancer, making Dok-2 and its interactions vital for understanding tumorigenesis and developing targeted therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dok-2 Antibody (E-10) References:
- Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases. | Mayer, BJ., et al. 1995. Curr Biol. 5: 296-305. PMID: 7780740
- Vascular endothelial cell growth factor promotes tyrosine phosphorylation of mediators of signal transduction that contain SH2 domains. Association with endothelial cell proliferation. | Guo, D., et al. 1995. J Biol Chem. 270: 6729-33. PMID: 7896817
- The IRS-1 signaling system. | Myers, MG., et al. 1994. Trends Biochem Sci. 19: 289-93. PMID: 8048169
- A 62-kilodalton tyrosine phosphoprotein constitutively present in primary chronic phase chronic myelogenous leukemia enriched lineage negative blast populations. | Wisniewski, D., et al. 1994. Leukemia. 8: 688-93. PMID: 8152267
- A Grb2-associated docking protein in EGF- and insulin-receptor signalling. | Holgado-Madruga, M., et al. 1996. Nature. 379: 560-4. PMID: 8596638
- p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. | Carpino, N., et al. 1997. Cell. 88: 197-204. PMID: 9008160
- Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok. | Yamanashi, Y. and Baltimore, D. 1997. Cell. 88: 205-11. PMID: 9008161
- Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins. | Di Cristofano, A., et al. 1998. J Biol Chem. 273: 4827-30. PMID: 9478921
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dok-2 Antibody (E-10) | sc-17830 | 200 µg/ml | $322.00 | |||
Dok-2 Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-528329 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dok-2 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-520675 | 200 µg Ab, 40 µg BP | $361.00 | |||
Dok-2 Antibody (E-10): m-IgG1 BP-HRP Bundle | sc-542869 | 200 µg Ab; 20 µg BP | $361.00 | |||
Dok-2 Antibody (E-10) AC | sc-17830 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Dok-2 Antibody (E-10) HRP | sc-17830 HRP | 200 µg/ml | $322.00 | |||
Dok-2 Antibody (E-10) FITC | sc-17830 FITC | 200 µg/ml | $336.00 | |||
Dok-2 Antibody (E-10) PE | sc-17830 PE | 200 µg/ml | $349.00 | |||
Dok-2 Antibody (E-10) Alexa Fluor® 488 | sc-17830 AF488 | 200 µg/ml | $364.00 | |||
Dok-2 Antibody (E-10) Alexa Fluor® 546 | sc-17830 AF546 | 200 µg/ml | $364.00 | |||
Dok-2 Antibody (E-10) Alexa Fluor® 594 | sc-17830 AF594 | 200 µg/ml | $364.00 | |||
Dok-2 Antibody (E-10) Alexa Fluor® 647 | sc-17830 AF647 | 200 µg/ml | $364.00 | |||
Dok-2 Antibody (E-10) Alexa Fluor® 680 | sc-17830 AF680 | 200 µg/ml | $364.00 | |||
Dok-2 Antibody (E-10) Alexa Fluor® 790 | sc-17830 AF790 | 200 µg/ml | $364.00 |