DOCK 180 Antibody (H-4): sc-13163

DOCK 180 Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects DOCK 180 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DOCK 180 monoclonal antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DOCK 180 plays a crucial role in cellular signaling pathways, particularly in the regulation of cytoskeletal dynamics and cell migration. DOCK 180 acts as a guanine nucleotide exchange factor (GEF) for the Rho family of GTPases, which are essential for actin cytoskeleton reorganization. This function is vital for processes such as cell adhesion, motility, and the formation of cellular structures like lamellipodia and filopodia. DOCK 180′s ability to modulate these processes underscores its importance in developmental biology and cancer metastasis, where altered cell migration can lead to tumor progression. By utilizing DOCK 180 monoclonal antibody (H-4), researchers can gain insights into the mechanisms of cell signaling and DOCK 180′s role in various physiological and pathological contexts.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DOCK 180 Antibody (H-4) References:

1. Biological and biochemical activity of v-Crk chimeras containing the SH2/SH3 regions of phosphatidylinositol-specific phospholipase C-gamma and Src.  |  Matsuda, M., et al. 1992. J Virol. 66: 115-21. PMID: 1370084
2. Dock180 and ELMO1 proteins cooperate to promote evolutionarily conserved Rac-dependent cell migration.  |  Grimsley, CM., et al. 2004. J Biol Chem. 279: 6087-97. PMID: 14638695
3. Two species of human CRK cDNA encode proteins with distinct biological activities.  |  Matsuda, M., et al. 1992. Mol Cell Biol. 12: 3482-9. PMID: 1630456
4. Dock180-ELMO cooperation in Rac activation.  |  Lu, M. and Ravichandran, KS. 2006. Methods Enzymol. 406: 388-402. PMID: 16472672
5. Elmo1 inhibits ubiquitylation of Dock180.  |  Makino, Y., et al. 2006. J Cell Sci. 119: 923-32. PMID: 16495483
6. Association of the v-crk oncogene product with phosphotyrosine-containing proteins and protein kinase activity.  |  Mayer, BJ. and Hanafusa, H. 1990. Proc Natl Acad Sci U S A. 87: 2638-42. PMID: 1690891
7. Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins.  |  Matsuda, M., et al. 1990. Science. 248: 1537-9. PMID: 1694307
8. Mutagenic analysis of the v-crk oncogene: requirement for SH2 and SH3 domains and correlation between increased cellular phosphotyrosine and transformation.  |  Mayer, BJ. and Hanafusa, H. 1990. J Virol. 64: 3581-9. PMID: 1695251
9. The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons.  |  Kim, JY., et al. 2011. J Biol Chem. 286: 37615-24. PMID: 21900250
10. Overexpression of Dock180 and Elmo1 in Melanoma is Associated with Cell Survival and Migration.  |  Lee, YJ., et al. 2023. Ann Dermatol. 35: 439-450. PMID: 38086358
11. Both the SH2 and SH3 domains of human CRK protein are required for neuronal differentiation of PC12 cells.  |  Tanaka, S., et al. 1993. Mol Cell Biol. 13: 4409-15. PMID: 8321240
12. DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane.  |  Hasegawa, H., et al. 1996. Mol Cell Biol. 16: 1770-6. PMID: 8657152

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DOCK 180 Antibody (H-4) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-DOCK 180 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DOCK 180 Antibody (H-4) Specifications

Alternate Product Name: DOCK 180

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1700-1769

Epitope Target: DOCK 180

Epitope Species: human

Product Reactivity/Detection: DOCK 180

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for DOCK 180 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred DOCK 180 antibody is DOCK 180 Antibody (E-2): sc-514080.