DOCK 180 Antibody (E-2): sc-514080

DOCK 180 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects DOCK180 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DOCK180 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DOCK180 protein plays a crucial role in cellular signaling and cytoskeletal dynamics, acting as a guanine nucleotide exchange factor (GEF) for Rac1, a member of the Rho family of GTPases. This function is vital for regulating actin cytoskeleton rearrangements, which are essential for processes such as cell migration, adhesion, and morphogenesis. DOCK180 interaction with Crk proteins facilitates DOCK180 recruitment to the plasma membrane, where DOCK180 can activate Rac1, leading to significant changes in cell shape and motility. Understanding DOCK180 role in these processes is important for elucidating mechanisms underlying various physiological and pathological conditions, including cancer metastasis and immune responses.

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DOCK 180 Antibody (E-2) References:

1. The DHR1 domain of DOCK180 binds to SNX5 and regulates cation-independent mannose 6-phosphate receptor transport.  |  Hara, S., et al. 2008. Mol Biol Cell. 19: 3823-35. PMID: 18596235
2. DOCK180 is a Rac activator that regulates cardiovascular development by acting downstream of CXCR4.  |  Sanematsu, F., et al. 2010. Circ Res. 107: 1102-5. PMID: 20829512
3. Activation of Rac1 by Src-dependent phosphorylation of Dock180(Y1811) mediates PDGFRα-stimulated glioma tumorigenesis in mice and humans.  |  Feng, H., et al. 2011. J Clin Invest. 121: 4670-84. PMID: 22080864
4. Overexpression of dedicator of cytokinesis I (Dock180) in ovarian cancer correlated with aggressive phenotype and poor patient survival.  |  Zhao, F., et al. 2011. Histopathology. 59: 1163-72. PMID: 22175896
5. Pro-survival effect of Dock180 overexpression on rat-derived H9C2 cardiomyocytes.  |  Yan, A., et al. 2013. Med Sci Monit Basic Res. 19: 12-9. PMID: 23314417
6. EGFRvIII stimulates glioma growth and invasion through PKA-dependent serine phosphorylation of Dock180.  |  Feng, H., et al. 2014. Oncogene. 33: 2504-12. PMID: 23728337
7. Elmo1 helps dock180 to regulate Rac1 activity and cell migration of ovarian cancer.  |  Wang, J., et al. 2014. Int J Gynecol Cancer. 24: 844-50. PMID: 24819662
8. Protein kinase A-dependent phosphorylation of Dock180 at serine residue 1250 is important for glioma growth and invasion stimulated by platelet derived-growth factor receptor α.  |  Feng, H., et al. 2015. Neuro Oncol. 17: 832-42. PMID: 25468898
9. Silencing of dedicator of cytokinesis (DOCK180) obliterates pregnancy by interfering with decidualization due to blockage of nuclear entry of autoimmune regulator (AIRE).  |  Mohan, JJ., et al. 2018. Am J Reprod Immunol. 80: e12844. PMID: 29516628
10. Dragon's blood attenuates LPS-induced intestinal epithelial barrier dysfunction via upregulation of FAK-DOCK180-Rac1-WAVE2-Arp3 and downregulation of TLR4/NF-κB signaling pathways.  |  Liu, H., et al. 2024. J Nat Med. 78: 1013-1028. PMID: 39014275

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DOCK 180 Antibody (E-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-DOCK 180 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DOCK 180 Antibody (E-2) Specifications

Alternate Product Name: DOCK1

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-24

Epitope Target: DOCK 180

Epitope Species: human

Product Reactivity/Detection: DOCK 180

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for DOCK 180 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred DOCK 180 antibody is DOCK 180 Antibody (H-4): sc-13163.