DNase I Antibody (D-1): sc-374207

DNase I Antibody (D-1) is a mouse monoclonal IgG1 antibody that detects DNase I in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNase I plays a crucial role in the apoptotic process by mediating internucleosomal DNA fragmentation, which is a hallmark of programmed cell death. This enzyme is a calcium and magnesium-dependent endonuclease that is ubiquitously expressed in mammalian tissues, highlighting its importance in cellular homeostasis and the regulation of apoptosis. Notably, DNase I is highly polymorphic, with at least six known alleles, which may influence its activity and function in different individuals. DNase I is primarily localized in the nuclei of epithelial and fiber cells, where DNase I cleaves DNA into 5′-phosphodinucleotide and 5′-phospho-oligonucleotide end-products, facilitating the orderly degradation of genomic DNA during apoptosis. Understanding the function and localization of DNase I is essential, as dysregulation of this enzyme can lead to various pathological conditions, including autoimmune diseases and cancer. Anti-DNase I antibody (D-1) is an invaluable tool for researchers studying apoptosis and related cellular processes.

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DNase I Antibody (D-1) References:

1. Renoprotective Effects of DNAse-I Treatment in a Rat Model of Ischemia/Reperfusion-Induced Acute Kidney Injury.  |  Peer, V., et al. 2016. Am J Nephrol. 43: 195-205. PMID: 27073834
2. DNase γ, DNase I and caspase-activated DNase cooperate to degrade dead cells.  |  Koyama, R., et al. 2016. Genes Cells. 21: 1150-1163. PMID: 27654959
3. DNase I improves corneal epithelial and nerve regeneration in diabetic mice.  |  Zhang, J., et al. 2020. J Cell Mol Med. 24: 4547-4556. PMID: 32168430
4. DNase I improves blood-milk barrier integrity and alleviates inflammation induced by Staphylococcus aureus during mastitis.  |  Jingjing, W., et al. 2021. Int Immunopharmacol. 100: 108079. PMID: 34455255
5. iDHS-FFLG: Identifying DNase I Hypersensitive Sites by Feature Fusion and Local-Global Feature Extraction Network.  |  Wang, LS. and Sun, ZL. 2023. Interdiscip Sci. 15: 155-170. PMID: 36166165
6. Hydrogel delivery of DNase I and liposomal vancomycin to eradicate fracture-related methicillin-resistant staphylococcus aureus infection and support osteoporotic fracture healing.  |  Li, J., et al. 2023. Acta Biomater. 164: 223-239. PMID: 37019168
7. Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration.  |  Bin, Z., et al. 2023. J Tradit Chin Med. 43: 661-666. PMID: 37454250
8. Genetically Engineered Cellular Nanovesicle as Targeted DNase I Delivery System for the Clearance of Neutrophil Extracellular Traps in Acute Lung Injury.  |  Du, Y., et al. 2023. Adv Sci (Weinh). 10: e2303053. PMID: 37759381
9. DNase I targeted degradation of neutrophil extracellular traps to reduce the damage on IgAV rat.  |  Chen, XQ., et al. 2023. PLoS One. 18: e0291592. PMID: 37906560
10. Extracellular G-quadruplexes and Z-DNA protect biofilms from DNase I, and G-quadruplexes form a DNAzyme with peroxidase activity.  |  Minero, GAS., et al. 2024. Nucleic Acids Res. 52: 1575-1590. PMID: 38296834

The preferred DNase I antibody is DNase I Antibody (B-4): sc-376207.