DNA pol µ Antibody (E-8): sc-398666
- DNA pol µ Antibody (E-8) is a mouse monoclonal IgG1 κ DNA pol µ antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 447-469 near the C-terminus of DNA Polymerase µ of human origin
- DNA pol µ Antibody (E-8) is recommended for detection of DNA Polymerase µ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DNA pol µ Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DNA pol µ Antibody (E-8) for WB applications. These reagents are now offered in bundles with DNA pol µ Antibody (E-8) (see ordering information below).
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DNA pol µ Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNA pol µ of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol µ antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA polymerase µ is particularly noteworthy for its unique ability to incorporate both deoxynucleotides and ribonucleotides during DNA synthesis, which distinguishes DNA polymerase µ from other DNA polymerases that typically exhibit substrate specificity for deoxynucleotides alone. This dual incorporation capability suggests that DNA polymerase µ may play a critical role in DNA repair processes, particularly in microhomology-mediated end joining, which is essential for the repair of double-stranded breaks. The presence of ribonucleotides in DNA can influence structural integrity and functionality of resulting DNA, making DNA polymerase µ and its interactions vital for understanding cellular responses to DNA damage and maintenance of genomic stability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DNA pol µ Antibody (E-8) References:
- Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair. | Mahajan, KN., et al. 2002. Mol Cell Biol. 22: 5194-202. PMID: 12077346
- Lesion bypass activities of human DNA polymerase mu. | Zhang, Y., et al. 2002. J Biol Chem. 277: 44582-7. PMID: 12228225
- DNA polymerase mu gene expression in B-cell non-Hodgkin's lymphomas: an analysis utilizing in situ hybridization. | Chiu, A., et al. 2002. Am J Pathol. 161: 1349-55. PMID: 12368208
- Translesion synthesis past platinum DNA adducts by human DNA polymerase mu. | Havener, JM., et al. 2003. Biochemistry. 42: 1777-88. PMID: 12578393
- Polymerase mu is a DNA-directed DNA/RNA polymerase. | Nick McElhinny, SA. and Ramsden, DA. 2003. Mol Cell Biol. 23: 2309-15. PMID: 12640116
- Lesion bypass by human DNA polymerase mu reveals a template-dependent, sequence-independent nucleotidyl transferase activity. | Covo, S., et al. 2004. J Biol Chem. 279: 859-65. PMID: 14581466
- Efficient and error-free replication past a minor-groove DNA adduct by the sequential action of human DNA polymerases iota and kappa. | Washington, MT., et al. 2004. Mol Cell Biol. 24: 5687-93. PMID: 15199127
- Overexpression of human DNA polymerase mu (Pol mu) in a Burkitt's lymphoma cell line affects the somatic hypermutation rate. | Ruiz, JF., et al. 2004. Nucleic Acids Res. 32: 5861-73. PMID: 15520469
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol µ Antibody (E-8) | sc-398666 | 200 µg/ml | $322.00 | |||
DNA pol µ Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-530894 | 200 µg Ab; 10 µg BP | $361.00 | |||
DNA pol µ Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-524445 | 200 µg Ab, 40 µg BP | $361.00 | |||
DNA pol µ Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544304 | 200 µg Ab; 20 µg BP | $361.00 | |||
DNA pol μ Antibody (E-8) AC | sc-398666 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DNA pol μ Antibody (E-8) HRP | sc-398666 HRP | 200 µg/ml | $322.00 | |||
DNA pol μ Antibody (E-8) FITC | sc-398666 FITC | 200 µg/ml | $336.00 | |||
DNA pol μ Antibody (E-8) PE | sc-398666 PE | 200 µg/ml | $349.00 | |||
DNA pol μ Antibody (E-8) Alexa Fluor® 488 | sc-398666 AF488 | 200 µg/ml | $364.00 | |||
DNA pol μ Antibody (E-8) Alexa Fluor® 546 | sc-398666 AF546 | 200 µg/ml | $364.00 | |||
DNA pol μ Antibody (E-8) Alexa Fluor® 594 | sc-398666 AF594 | 200 µg/ml | $364.00 | |||
DNA pol μ Antibody (E-8) Alexa Fluor® 647 | sc-398666 AF647 | 200 µg/ml | $364.00 | |||
DNA pol μ Antibody (E-8) Alexa Fluor® 680 | sc-398666 AF680 | 200 µg/ml | $364.00 | |||
DNA pol μ Antibody (E-8) Alexa Fluor® 790 | sc-398666 AF790 | 200 µg/ml | $364.00 | |||
DNA pol μ (E-8) Neutralizing Peptide | sc-398666 P | 100 µg/0.5 ml | $69.00 |