DNA pol ε B Antibody (C-9): sc-398582

DNA pol ε B Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNA pol epsilon B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol epsilon B antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA pol epsilon B protein plays a crucial role in DNA replication, particularly in the synthesis of the leading strand, where DNA pol epsilon B ensures high-fidelity DNA synthesis and contributes to overall genome stability. DNA pol epsilon B protein functions as part of a multi-subunit complex, with its catalytic subunit encoded by the POL2 gene, which is essential for accurate replication of genetic material. Proper functioning of DNA pol epsilon B protein is vital, as replication errors can lead to mutations and genomic instability, which are hallmarks of various diseases, including cancer. Understanding DNA pol epsilon B protein interactions and mechanisms not only sheds light on fundamental biological processes but also opens avenues for therapeutic interventions targeting DNA replication pathways.

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DNA pol ε B Antibody (C-9) References:

1. Direct interaction of proliferating cell nuclear antigen with the p125 catalytic subunit of mammalian DNA polymerase delta.  |  Zhang, P., et al. 1999. J Biol Chem. 274: 26647-53. PMID: 10480866
2. A third essential DNA polymerase in S. cerevisiae.  |  Morrison, A., et al. 1990. Cell. 62: 1143-51. PMID: 2169349
3. Discontinuous DNA synthesis by purified mammalian proteins.  |  Goulian, M., et al. 1990. J Biol Chem. 265: 18461-71. PMID: 2170412
4. Defect of Dpb2p, a noncatalytic subunit of DNA polymerase ɛ, promotes error prone replication of undamaged chromosomal DNA in Saccharomyces cerevisiae.  |  Kraszewska, J., et al. 2012. Mutat Res. 737: 34-42. PMID: 22709919
5. Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.  |  Hamatake, RK., et al. 1990. J Biol Chem. 265: 4072-83. PMID: 2406268
6. Gap-directed translesion DNA synthesis of an abasic site on circular DNA templates by a human replication complex.  |  Villani, G., et al. 2014. PLoS One. 9: e93908. PMID: 24710081
7. DNA polymerase ε and its roles in genome stability.  |  Henninger, EE. and Pursell, ZF. 2014. IUBMB Life. 66: 339-51. PMID: 24861832
8. Structural insights into eukaryotic DNA replication.  |  Doublié, S. and Zahn, KE. 2014. Front Microbiol. 5: 444. PMID: 25202305
9. A conserved Polϵ binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1.  |  García-Rodríguez, LJ., et al. 2015. Nucleic Acids Res. 43: 8830-8. PMID: 26250113
10. Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.  |  Lee, MY., et al. 1984. Biochemistry. 23: 1906-13. PMID: 6426510
11. Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA.  |  Johnson, RE., et al. 1995. Science. 269: 238-40. PMID: 7618086
12. Regulation of human DNA polymerase delta during the cell cycle.  |  Zeng, XR., et al. 1994. J Biol Chem. 269: 24027-33. PMID: 7929054

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA pol ε B Antibody (C-9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA pol ε B Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA pol ε B Antibody (C-9) Specifications

Alternate Product Name: DNA pol ε B

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: DNA pol ε B

Epitope Species: human

Product Reactivity/Detection: DNA pol ε B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DNA pol ε B Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin