DNA pol δ cat Antibody (E-3): sc-55499

DNA pol δ cat Antibody (E-3) is a mouse monoclonal IgG2a antibody that detects DNA polymerase delta (DNA pol δ) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA pol δ plays a crucial role in DNA replication, recombination, and repair, which are essential processes for maintaining genome stability. Anti-DNA pol δ antibody (E-3) is particularly useful for studying the synthesis of the lagging strand during DNA replication, where DNA pol δ processes Okazaki fragments, ensuring that the newly synthesized DNA is accurate and complete. The structure of DNA pol δ is composed of multiple subunits, including the p125 and p50 subunits, with p125 directly interacting with the sliding DNA clamp protein PCNA, facilitating its function during replication. Understanding the structure of DNA pol δ provides insights into how the enzyme is regulated by various cell cycle proteins and how DNA pol δ interacts with other components of the DNA replication machinery. The precise mechanisms by which DNA pol δ operates on the leading and lagging strands remain an area of active research, highlighting the significance of DNA pol δ monoclonal antibody (E-3) in studying the dynamics of DNA replication and repair pathways.

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DNA pol δ cat Antibody (E-3) References:

1. Characterization of Mycobacterium smegmatis PolD2 and PolD1 as RNA/DNA polymerases homologous to the POL domain of bacterial DNA ligase D.  |  Zhu, H., et al. 2012. Biochemistry. 51: 10147-58. PMID: 23198659
2. Human POLD1 modulates cell cycle progression and DNA damage repair.  |  Song, J., et al. 2015. BMC Biochem. 16: 14. PMID: 26087769
3. Elevated expression of POLD1 is associated with poor prognosis in breast cancer.  |  Qin, Q., et al. 2018. Oncol Lett. 16: 5591-5598. PMID: 30344713
4. E2F1 mediates the downregulation of POLD1 in replicative senescence.  |  Gao, S., et al. 2019. Cell Mol Life Sci. 76: 2833-2850. PMID: 30895337
5. Assessment of POLE and POLD1 mutations as prognosis and immunotherapy biomarkers for stomach adenocarcinoma.  |  Zhu, M., et al. 2022. Transl Cancer Res. 11: 193-205. PMID: 35261896
6. POLD1 as a Prognostic Biomarker Correlated with Cell Proliferation and Immune Infiltration in Clear Cell Renal Cell Carcinoma.  |  Tian, J., et al. 2023. Int J Mol Sci. 24: PMID: 37047824
7. Rare germline variants in POLE and POLD1 encoding the catalytic subunits of DNA polymerases ε and δ in glioma families.  |  Weber, CAM., et al. 2023. Acta Neuropathol Commun. 11: 184. PMID: 37990341
8. POLD1 DEDD Motif Mutation Confers Hypermutation in Endometrial Cancer and Durable Response to Pembrolizumab.  |  Wei, CH., et al. 2023. Cancers (Basel). 15: PMID: 38067377
9. Altered immunoexpression of DNA polymerase delta 1 catalytic subunit (POLD1) in colorectal cancer.  |  Stefaniak, P., et al. 2023. Contemp Oncol (Pozn). 27: 147-154. PMID: 38239863
10. Proofreading the way: immune checkpoint inhibitors in polymerase ε/polymerase δ (POLE/POLD1)-altered colorectal cancer.  |  Cecchini, M. and Sundar, R. 2024. Ann Oncol. 35: 582-584. PMID: 38910015

The preferred DNA pol δ cat antibody is DNA pol δ cat Antibody (A-9): sc-17776.