DLC-1 Antibody (C-12): sc-271915

DLC-1 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects DLC-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DLC-1 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DLC-1, also known as Deleted In Liver Cancer 1, plays a crucial role as a Rho GTPase-activating protein, which is essential for regulating cell growth and motility. Loss of DLC-1 expression is frequently associated with tumor progression in various human tissues, including breast, liver, colon, and prostate. The gene encoding DLC-1 is located on human chromosome 8p21-22, a region often linked to tumor suppressor genes due to frequent mutations in numerous cancers. DLC-1 is primarily localized in the cytoplasm, and restoration of DLC-1 has been shown to induce apoptosis via caspase-3 activation, thereby inhibiting cell growth and invasiveness, making DLC-1 a significant target for cancer research and therapeutic strategies aimed at restoring function in malignancies.

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DLC-1 Antibody (C-12) References:

1. DLC-1 gene inhibits human breast cancer cell growth and in vivo tumorigenicity.  |  Yuan, BZ., et al. 2003. Oncogene. 22: 445-50. PMID: 12545165
2. DNA variants of DLC-1, a candidate tumor suppressor gene in human hepatocellular carcinoma.  |  Park, SW., et al. 2003. Int J Oncol. 23: 133-7. PMID: 12792785
3. Restoration of DLC-1 gene expression induces apoptosis and inhibits both cell growth and tumorigenicity in human hepatocellular carcinoma cells.  |  Zhou, X., et al. 2004. Oncogene. 23: 1308-13. PMID: 14647417
4. DLC-1 operates as a tumor suppressor gene in human non-small cell lung carcinomas.  |  Yuan, BZ., et al. 2004. Oncogene. 23: 1405-11. PMID: 14661059
5. Identification of ATF-3, caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene profiling.  |  Syed, V., et al. 2005. Oncogene. 24: 1774-87. PMID: 15674352
6. DLC-1:a Rho GTPase-activating protein and tumour suppressor.  |  Durkin, ME., et al. 2007. J Cell Mol Med. 11: 1185-207. PMID: 17979893
7. Role of DLC-1, a tumor suppressor protein with RhoGAP activity, in regulation of the cytoskeleton and cell motility.  |  Kim, TY., et al. 2009. Cancer Metastasis Rev. 28: 77-83. PMID: 19221866
8. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway.  |  Huang, W., et al. 2015. Med Oncol. 32: 115. PMID: 25779535
9. Enhanced p122RhoGAP/DLC-1 Expression Can Be a Cause of Coronary Spasm.  |  Kinjo, T., et al. 2015. PLoS One. 10: e0143884. PMID: 26624289
10. Stiffness-Induced Endothelial DLC-1 Expression Forces Leukocyte Spreading through Stabilization of the ICAM-1 Adhesome.  |  Schimmel, L., et al. 2018. Cell Rep. 24: 3115-3124. PMID: 30231995
11. DLC-1 tumor suppressor regulates CD105 expression on human non-small cell lung carcinoma cells through inhibiting TGF-β1 signaling.  |  Zhang, K., et al. 2020. Exp Cell Res. 386: 111732. PMID: 31770531
12. Cloning, characterization, and chromosomal localization of a gene frequently deleted in human liver cancer (DLC-1) homologous to rat RhoGAP.  |  Yuan, BZ., et al. 1998. Cancer Res. 58: 2196-9. PMID: 9605766

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DLC-1 Antibody (C-12) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-DLC-1 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DLC-1 Antibody (C-12) Specifications

Alternate Product Name: DLC1

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 111-370

Epitope Target: DLC-1

Epitope Species: human

Product Reactivity/Detection: DLC-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for DLC-1 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin