DISC-1 Antibody (B-2): sc-365591

DISC-1 Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects DISC-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DISC-1 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DISC-1 protein, encoded by the "Disrupted In Schizophrenia" gene locus, plays a crucial role in neuronal development and function, particularly in processes such as intracellular transport and neuronal migration, which are essential for proper brain architecture and signaling. DISC-1 protein interacts with several key proteins, including MIPT3, MAP1A, and nudel, which are critical for centrosome and cytoskeletal organization, as well as α-actinin-2 and spectrin β IV, which are important for receptor localization to membranes. DISC-1 protein is integral to maintaining neuronal integrity and function, and dysregulation is associated with schizophrenia, as evidenced by altered localization in mutant forms that predominantly reside in the cytoplasm rather than the nucleus. DISC-1 protein interactions with various signaling proteins, such as ATF-4 and ATF-5, further underscore its importance in transducing signals from membrane receptors, thereby influencing neuronal health and behavior.

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DISC-1 Antibody (B-2) References:

1. Disrupted-in-Schizophrenia-1 (DIsc-1): mutant truncation prevents binding to NudE-like (NUDEL) and inhibits neurite outgrowth.  |  Ozeki, Y., et al. 2003. Proc Natl Acad Sci U S A. 100: 289-94. PMID: 12506198
2. DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation.  |  Morris, JA., et al. 2003. Hum Mol Genet. 12: 1591-608. PMID: 12812986
3. Disrupted-In-Schizophrenia 1, a candidate gene for schizophrenia, participates in neurite outgrowth.  |  Miyoshi, K., et al. 2003. Mol Psychiatry. 8: 685-94. PMID: 12874605
4. Disrupted in Schizophrenia 1 and Nudel form a neurodevelopmentally regulated protein complex: implications for schizophrenia and other major neurological disorders.  |  Brandon, NJ., et al. 2004. Mol Cell Neurosci. 25: 42-55. PMID: 14962739
5. Expression of disrupted in schizophrenia 1 (DISC1) protein in the adult and developing mouse brain indicates its role in neurodevelopment.  |  Schurov, IL., et al. 2004. Mol Psychiatry. 9: 1100-10. PMID: 15381924
6. Inhibition of NUDEL (nuclear distribution element-like)-oligopeptidase activity by disrupted-in-schizophrenia 1.  |  Hayashi, MA., et al. 2005. Proc Natl Acad Sci U S A. 102: 3828-33. PMID: 15728732
7. Subcellular targeting of DISC1 is dependent on a domain independent from the Nudel binding site.  |  Brandon, NJ., et al. 2005. Mol Cell Neurosci. 28: 613-24. PMID: 15797709
8. Disrupted-In-Schizophrenia-1 (DISC1) interactome and mental disorders: impact of mouse models.  |  Lipina, TV. and Roder, JC. 2014. Neurosci Biobehav Rev. 45: 271-94. PMID: 25016072
9. Role of DISC1 in Neuronal Trafficking and its Implication in Neuropsychiatric Manifestation and Neurotherapeutics.  |  Tomoda, T., et al. 2017. Neurotherapeutics. 14: 623-629. PMID: 28664299
10. Gene polymorphisms of DISC1 is associated with schizophrenia: Evidence from a meta-analysis.  |  Wang, HY., et al. 2018. Prog Neuropsychopharmacol Biol Psychiatry. 81: 64-73. PMID: 29031911
11. Polysialylation in a DISC1 Mutant Mouse.  |  Takahashi, Y., et al. 2022. Int J Mol Sci. 23: PMID: 35563598
12. Astrocyte-mediated regulation of BLAWFS1 neurons alleviates risk-assessment deficits in DISC1-N mice.  |  Zhou, X., et al. 2024. Neuron. 112: 2197-2217.e7. PMID: 38642554

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DISC-1 Antibody (B-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-DISC-1 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DISC-1 Antibody (B-2) Specifications

Alternate Product Name: Disrupted In Schizophrenia 1

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 601-810

Epitope Target: DISC-1

Epitope Species: human

Product Reactivity/Detection: DISC-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for DISC-1 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin