DIS3L Antibody (E-12): sc-398739
- DIS3L Antibody (E-12) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 581-690 mapping within an internal region of DIS3L of human origin
- Anti-DIS3L Antibody (E-12) is recommended for detection of DIS3L of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DIS3L Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DIS3L Antibody (E-12) for WB applications. These reagents are now offered in bundles with DIS3L Antibody (E-12) (see ordering information below).
DIS3L Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects DIS3L in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DIS3L (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. DIS3L, a 1,054 amino acid exonuclease belonging to the ribonuclease II (RNB) family, plays a crucial role in RNA metabolism by participating in the degradation of unstable mRNAs that contain AU-rich elements in their untranslated 3′ regions. This function is vital for maintaining cellular homeostasis and regulating gene expression, as the timely degradation of these mRNAs prevents the accumulation of potentially harmful transcripts. DIS3L exists in three alternatively spliced isoforms, which may have distinct regulatory roles in the processing of pre-mRNA into mature mRNA. The gene encoding DIS3L is located on human chromosome 15, a region associated with several genetic disorders, including Angelman syndrome and Prader-Willi syndrome, highlighting the importance of this protein in both normal cellular function and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DIS3L Antibody (E-12) References:
- The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements. | Mukherjee, D., et al. 2002. EMBO J. 21: 165-74. PMID: 11782436
- Autoantibodies directed to novel components of the PM/Scl complex, the human exosome. | Brouwer, R., et al. 2002. Arthritis Res. 4: 134-8. PMID: 11879549
- Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring. | Raijmakers, R., et al. 2002. J Mol Biol. 323: 653-63. PMID: 12419256
- The association of the human PM/Scl-75 autoantigen with the exosome is dependent on a newly identified N terminus. | Raijmakers, R., et al. 2003. J Biol Chem. 278: 30698-704. PMID: 12788944
- Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis. | Schilders, G., et al. 2007. Arthritis Res Ther. 9: R12. PMID: 17280603
- Overexpression of the C-type natriuretic peptide (CNP) is associated with overgrowth and bone anomalies in an individual with balanced t(2;7) translocation. | Bocciardi, R., et al. 2007. Hum Mutat. 28: 724-31. PMID: 17373680
- Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways. | van Dijk, EL., et al. 2007. RNA. 13: 1027-35. PMID: 17545563
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DIS3L Antibody (E-12) | sc-398739 | 200 µg/ml | $322.00 | |||
DIS3L Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-530910 | 200 µg Ab; 10 µg BP | $361.00 | |||
DIS3L Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-524464 | 200 µg Ab, 40 µg BP | $361.00 | |||
DIS3L Antibody (E-12): m-IgG2a BP-HRP Bundle | sc-547625 | 200 µg Ab; 10 µg BP | $361.00 | |||
DIS3L Antibody (E-12) AC | sc-398739 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DIS3L Antibody (E-12) HRP | sc-398739 HRP | 200 µg/ml | $322.00 | |||
DIS3L Antibody (E-12) FITC | sc-398739 FITC | 200 µg/ml | $336.00 | |||
DIS3L Antibody (E-12) PE | sc-398739 PE | 200 µg/ml | $349.00 | |||
DIS3L Antibody (E-12) Alexa Fluor® 488 | sc-398739 AF488 | 200 µg/ml | $364.00 | |||
DIS3L Antibody (E-12) Alexa Fluor® 546 | sc-398739 AF546 | 200 µg/ml | $364.00 | |||
DIS3L Antibody (E-12) Alexa Fluor® 594 | sc-398739 AF594 | 200 µg/ml | $364.00 | |||
DIS3L Antibody (E-12) Alexa Fluor® 647 | sc-398739 AF647 | 200 µg/ml | $364.00 | |||
DIS3L Antibody (E-12) Alexa Fluor® 680 | sc-398739 AF680 | 200 µg/ml | $364.00 | |||
DIS3L Antibody (E-12) Alexa Fluor® 790 | sc-398739 AF790 | 200 µg/ml | $364.00 |