DIS3 Antibody (H-3): sc-398663

DIS3 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects DIS3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DIS3 Antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DIS3, also known as RRP44, is a crucial component of the exosome complex, which plays a vital role in RNA metabolism by degrading unstable mRNAs that contain AU-rich elements in their untranslated regions. This degradation process is essential for maintaining cellular homeostasis and regulating gene expression, as it prevents the accumulation of potentially harmful mRNAs. DIS3 is a 958 amino acid protein that is widely expressed, with the highest levels found in the testis, and contains a PINc domain that is critical for exoribonuclease activity. DIS3 is localized in both the cytoplasm and nucleus, where DIS3 is involved in the processing of 7S pre-RNA into a mature nuclear complex, thereby ensuring proper mitotic progression. Dysregulation of DIS3 expression has been implicated in colon cancer, highlighting DIS3′s potential role in tumorigenesis. Additionally, multiple isoforms of DIS3 arise from alternative splicing events, which may contribute to functional diversity in various cellular contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DIS3 Antibody (H-3) References:

1. Three novel components of the human exosome.  |  Brouwer, R., et al. 2001. J Biol Chem. 276: 6177-84. PMID: 11110791
2. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.  |  Chen, CY., et al. 2001. Cell. 107: 451-64. PMID: 11719186
3. A protein interaction framework for human mRNA degradation.  |  Lehner, B. and Sanderson, CM. 2004. Genome Res. 14: 1315-23. PMID: 15231747
4. Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function.  |  Murakami, H., et al. 2007. PLoS One. 2: e317. PMID: 17380189
5. The exosome subunit Rrp44 plays a direct role in RNA substrate recognition.  |  Schneider, C., et al. 2007. Mol Cell. 27: 324-331. PMID: 17643380
6. Global role for polyadenylation-assisted nuclear RNA degradation in posttranscriptional gene silencing.  |  Wang, SW., et al. 2008. Mol Cell Biol. 28: 656-65. PMID: 18025105
7. New insights into the mechanism of RNA degradation by ribonuclease II: identification of the residue responsible for setting the RNase II end product.  |  Barbas, A., et al. 2008. J Biol Chem. 283: 13070-6. PMID: 18337246
8. Human dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3.  |  Shiomi, T., et al. 1998. J Biochem. 123: 883-90. PMID: 9562621

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DIS3 Antibody (H-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DIS3 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DIS3 Antibody (H-3) Specifications

Alternate Product Name: DIS3

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 788-958

Epitope Target: DIS3

Epitope Species: human

Product Reactivity/Detection: DIS3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DIS3 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin