DIO1 Antibody (B-7): sc-515198
- DIO1 Antibody (B-7) is a mouse monoclonal IgG2b κ DIO1 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 78-99 within an internal region of DIO1 of mouse origin
- DIO1 Antibody (B-7) is recommended for detection of DIO1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DIO1 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for DIO1 Antibody (B-7) for WB applications. This reagent is now offered in a bundle with DIO1 Antibody (B-7) (see ordering information below).
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DIO1 Antibody (B-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects DIO1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DIO1 Antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DIO1 (B-7) antibody targets deiodinase, iodothyronine, type I, a 249 amino acid single-pass membrane protein primarily localized to the endoplasmic reticulum and belonging to the iodothyronine deiodinase family. DIO1 (B-7) monoclonal antibody recognizes a protein crucial for thyroid hormone metabolism, functioning as a thiol-dependent propylthiouracil-sensitive oxidoreductase that converts thyroxine (T4) into bioactive 3,3′,5-triiodothyronine (T3), playing a significant role in thyroid hormone activation essential for regulating metabolism, growth, and development. DIO1 exists in nine alternatively spliced isoforms, with its gene located on human chromosome 1, containing over 3,000 genes and comprising nearly 8% of the human genome. The 88% sequence similarity between human DIO1 and rat counterpart underscores evolutionary conservation across species, making DIO1 a valuable target for thyroid hormone regulation research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DIO1 Antibody (B-7) References:
- In vivo dimerization of types 1, 2, and 3 iodothyronine selenodeiodinases. | Curcio-Morelli, C., et al. 2003. Endocrinology. 144: 937-46. PMID: 12586771
- Cloning and in vitro expression of the human selenoprotein, type I iodothyronine deiodinase. | Mandel, SJ., et al. 1992. J Clin Endocrinol Metab. 75: 1133-9. PMID: 1400883
- Gene expression profiles reveal that DCN, DIO1, and DIO2 are underexpressed in benign and malignant thyroid tumors. | Arnaldi, LA., et al. 2005. Thyroid. 15: 210-21. PMID: 15785240
- Regulation of type 1 iodothyronine deiodinase in health and disease. | Koenig, RJ. 2005. Thyroid. 15: 835-40. PMID: 16131326
- A common variation in deiodinase 1 gene DIO1 is associated with the relative levels of free thyroxine and triiodothyronine. | Panicker, V., et al. 2008. J Clin Endocrinol Metab. 93: 3075-81. PMID: 18492748
- Common variation in the DIO2 gene predicts baseline psychological well-being and response to combination thyroxine plus triiodothyronine therapy in hypothyroid patients. | Panicker, V., et al. 2009. J Clin Endocrinol Metab. 94: 1623-9. PMID: 19190113
- Topological analysis of the integral membrane protein, type 1 iodothyronine deiodinase (D1). | Toyoda, N., et al. 1995. J Biol Chem. 270: 12310-8. PMID: 7744884
- Activation and inactivation of thyroid hormone by type I iodothyronine deiodinase. | Moreno, M., et al. 1994. FEBS Lett. 344: 143-6. PMID: 8187873
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DIO1 Antibody (B-7) | sc-515198 | 200 µg/ml | $322.00 | |||
DIO1 Antibody (B-7): m-IgG2b BP-HRP Bundle | sc-548813 | 200 µg Ab; 10 µg BP | $361.00 | |||
DIO1 Antibody (B-7) AC | sc-515198 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DIO1 Antibody (B-7) HRP | sc-515198 HRP | 200 µg/ml | $322.00 | |||
DIO1 Antibody (B-7) FITC | sc-515198 FITC | 200 µg/ml | $336.00 | |||
DIO1 Antibody (B-7) PE | sc-515198 PE | 200 µg/ml | $349.00 | |||
DIO1 Antibody (B-7) Alexa Fluor® 488 | sc-515198 AF488 | 200 µg/ml | $364.00 | |||
DIO1 Antibody (B-7) Alexa Fluor® 546 | sc-515198 AF546 | 200 µg/ml | $364.00 | |||
DIO1 Antibody (B-7) Alexa Fluor® 594 | sc-515198 AF594 | 200 µg/ml | $364.00 | |||
DIO1 Antibody (B-7) Alexa Fluor® 647 | sc-515198 AF647 | 200 µg/ml | $364.00 | |||
DIO1 Antibody (B-7) Alexa Fluor® 680 | sc-515198 AF680 | 200 µg/ml | $364.00 | |||
DIO1 Antibody (B-7) Alexa Fluor® 790 | sc-515198 AF790 | 200 µg/ml | $364.00 | |||
DIO1 (B-7) Neutralizing Peptide | sc-515198 P | 100 µg/0.5 ml | $69.00 |