Dia 2 Antibody (B-11): sc-55539
- Dia 2 Antibody (B-11) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
- raised against mino acids 23-110 mapping near the N-terminus of Dia 2 of human origin
- recommended for detection of Dia 2 (also designated mDia3 of mouse origin) of mouse, rat and human origin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Dia 2 Antibody (B-11). This work is in progress.
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Dia 2 Antibody (B-11) is a mouse monoclonal IgG2b antibody that detects Dia 2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Dia 2, also known as DIAPH2 or mDia3, is a member of the formin homology protein family, which plays a crucial role in regulating actin filament dynamics. Dia 2 is primarily expressed in testis and ovary, where Dia 2 is essential for oogenesis, the process of egg development. The presence of a diaphanous autoregulatory domain (DAD) allows Dia 2 to regulate activity through autoinhibition, while the GTPase-binding domain (GBD/FH3) facilitates interactions with Rho GTPases, which are vital for cytoskeletal organization and cellular signaling. Notably, defects in the gene encoding Dia 2 have been linked to premature ovarian failure type 2A, a condition that results in diminished ovarian function and can significantly impact fertility. Additionally, Dia 2 exists in three alternatively spliced isoforms, which may contribute to functional diversity and regulatory mechanisms within different cellular contexts. With a critical role in actin dynamics and reproductive health, anti-Dia 2 antibody (B-11) serves as a valuable tool for researchers investigating molecular pathways involved in these processes.
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Dia 2 Antibody (B-11) References:
- Activation of the S-phase checkpoint inhibits degradation of the F-box protein Dia2. | Kile, AC. and Koepp, DM. 2010. Mol Cell Biol. 30: 160-71. PMID: 19858292
- The amino-terminal TPR domain of Dia2 tethers SCF(Dia2) to the replisome progression complex. | Morohashi, H., et al. 2009. Curr Biol. 19: 1943-9. PMID: 19913425
- Dia2 controls transcription by mediating assembly of the RSC complex. | Andress, EJ., et al. 2011. PLoS One. 6: e21172. PMID: 21701592
- The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. | Kim, DH., et al. 2012. J Biol Chem. 287: 44212-20. PMID: 23129771
- DIA-2, a polyherbal formulation ameliorates hyperglycemia and protein-oxidation without increasing the body weight in type II diabetic rats. | Kesavanarayanan, KS., et al. 2013. Eur Rev Med Pharmacol Sci. 17: 356-69. PMID: 23426539
- Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. | Maculins, T., et al. 2015. Curr Biol. 25: 2254-9. PMID: 26255844
- Herbal formulation, DIA-2 and Rosiglitazone ameliorates hyperglycemia and hepatic steatosis in type 2 diabetic rats. | Kesavanarayanan, KS., et al. 2015. Eur Rev Med Pharmacol Sci. 19: 3107-17. PMID: 26367735
- An interpretation of human diaphorase isozymes in terms of three gene loci DIA1, DIA2 and DIA3. | Fisher, RA., et al. 1977. Ann Hum Genet. 41: 139-49. PMID: 563699
- Erythrocyte diaphorases DIA1 and DIA2 in bloodstains. | Dowd, R. and Tumosa, CS. 1983. Z Rechtsmed. 91: 123-7. PMID: 6689390
- Underexpression of a novel gene, dia2, impairs the transition of Dictyostelium cells from growth to differentiation. | Chae, SC., et al. 1998. Biochem Biophys Res Commun. 252: 278-83. PMID: 9813183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dia 2 Antibody (B-11) | sc-55539 | 200 µg/ml | $316.00 |