DHRS6 Antibody (E-4): sc-393030

DHRS6 Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects DHRS6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DHRS6 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DHRS6 protein, also known as dehydrogenase/reductase SDR family member 6, EFA6R, SDR15C1, UCPA-OR, UNQ6308, or BDH2, is a 245 amino acid cytoplasmic protein that belongs to the short-chain dehydrogenases/reductases (SDR) family, which is an evolutionarily conserved group of oxidoreductases found across all forms of life. One of the critical aspects of DHRS6 is its role in the peripheral utilization of 3-hydroxybutyrate, a key metabolite in energy metabolism, particularly during periods of fasting or prolonged exercise. Cytoplasmic localization of DHRS6, along with its high ratio of oxidized NAD+, enhances DHRS6′s ability to convert elevated levels of circulating 3-hydroxybutyrate into acetoacetate, thereby facilitating energy production and maintaining metabolic homeostasis. Two alternatively spliced isoforms of DHRS6 further underscore DHRS6′s functional diversity and potential significance in cellular survival and metabolic regulation.

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DHRS6 Antibody (E-4) References:

1. D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme.  |  Ito, K., et al. 2006. J Mol Biol. 355: 722-33. PMID: 16325199
2. Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase.  |  Guo, K., et al. 2006. J Biol Chem. 281: 10291-7. PMID: 16380372
3. Characterization of human DHRS4: an inducible short-chain dehydrogenase/reductase enzyme with 3beta-hydroxysteroid dehydrogenase activity.  |  Matsunaga, T., et al. 2008. Arch Biochem Biophys. 477: 339-47. PMID: 18571493
4. Alternative transcription initiation and splicing variants of the DHRS4 gene cluster.  |  Zhang, Q., et al. 2009. Biosci Rep. 29: 47-56. PMID: 18754758
5. Comparison of predicted and experimental subcellular localization of two putative rat steroid dehydrogenases from the short-chain dehydrogenase/reductase protein superfamily.  |  Keller, B., et al. 2009. Mol Cell Endocrinol. 301: 43-6. PMID: 18775470
6. Chinese hamster monomeric carbonyl reductases of the short-chain dehydrogenase/reductase superfamily.  |  Miura, T., et al. 2009. Chem Biol Interact. 178: 110-6. PMID: 18983989
7. Medium- and short-chain dehydrogenase/reductase gene and protein families: Medium-chain and short-chain dehydrogenases/reductases in retinoid metabolism.  |  Parés, X., et al. 2008. Cell Mol Life Sci. 65: 3936-49. PMID: 19011747
8. Molecular determinants for the stereospecific reduction of 3-ketosteroids and reactivity towards all-trans-retinal of a short-chain dehydrogenase/reductase (DHRS4).  |  Endo, S., et al. 2009. Arch Biochem Biophys. 481: 183-90. PMID: 19056333
9. Closed complex of the D-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor.  |  Nakashima, K., et al. 2009. J Biochem. 145: 467-79. PMID: 19122202
10. Downregulation of BDH2 modulates iron homeostasis and promotes DNA demethylation in CD4+ T cells of systemic lupus erythematosus.  |  Zhao, M., et al. 2018. Clin Immunol. 187: 113-121. PMID: 29113828
11. BDH2 is downregulated in hepatocellular carcinoma and acts as a tumor suppressor regulating cell apoptosis and autophagy.  |  Liang, H., et al. 2019. J Cancer. 10: 3735-3745. PMID: 31333791
12. Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus.  |  Gao, X., et al. 2022. J Clin Invest. 132: PMID: 35499082

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DHRS6 Antibody (E-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-DHRS6 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DHRS6 Antibody (E-4) Specifications

Alternate Product Name: DHRS6

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 26-59

Epitope Target: DHRS6

Epitope Species: human

Product Reactivity/Detection: DHRS6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for DHRS6 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin