DHODH Antibody (D-6): sc-166377

DHODH Antibody (D-6) is a mouse monoclonal IgG1 antibody that detects dihydroorotate dehydrogenase (DHODH) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DHODH is a crucial mitochondrial protein composed of 395 amino acids, located on the outer surface of the inner mitochondrial membrane, where DHODH plays a vital role in the fourth step of de novo pyrimidine biosynthesis. This metabolic pathway is essential for nucleic acid synthesis, making DHODH a significant target for various cancer chemotherapy agents. Additionally, DHODH is intricately linked to the respiratory chain, facilitating the transfer of electrons to ubiquinone, which is critical for cellular respiration and energy production. DHODH features a bipartite signal at its N-terminus that governs translocation into the mitochondrial inner membrane, underscoring the importance of proper localization for optimal function. Notably, DHODH activity can be inhibited by nitric oxide, which indirectly affects cytochrome c oxidase, leading to immunosuppressive and antiproliferative effects relevant to conditions such as rheumatoid arthritis. With multifaceted roles in metabolism and disease, anti-DHODH antibody (D-6) serves as a valuable tool for researchers investigating DHODH implications in various biological contexts.

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DHODH Antibody (D-6) References:

1. 17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy.  |  Tian, Y., et al. 2023. Redox Biol. 62: 102708. PMID: 37116254
2. Menaquinone-4 attenuates ferroptosis by upregulating DHODH through activation of SIRT1 after subarachnoid hemorrhage.  |  Zhang, J., et al. 2024. Free Radic Biol Med. 210: 416-429. PMID: 38042225
3. DHODH inhibition represents a therapeutic strategy and improves abiraterone treatment in castration-resistant prostate cancer.  |  Guo, S., et al. 2024. Oncogene. 43: 1399-1410. PMID: 38480915
4. Cirbp suppression compromises DHODH-mediated ferroptosis defense and attenuates hypothermic cardioprotection in an aged donor transplantation model.  |  Zhu, Y., et al. 2024. J Clin Invest. 134: PMID: 38690728
5. Bioinformatics analysis and experimental verification of the cancer-promoting effect of DHODH in clear cell renal cell carcinoma.  |  Wang, S., et al. 2024. Sci Rep. 14: 11985. PMID: 38796629
6. A targetable PRR11-DHODH axis drives ferroptosis- and temozolomide-resistance in glioblastoma.  |  Miao, Z., et al. 2024. Redox Biol. 73: 103220. PMID: 38838551
7. DHODH inhibition enhances the efficacy of immune checkpoint blockade by increasing cancer cell antigen presentation.  |  Mullen, NJ., et al. 2024. Elife. 12: PMID: 38973593
8. Mitochondrial Dysfunction-Evoked DHODH Acetylation is Involved in Renal Cell Ferroptosis during Cisplatin-Induced Acute Kidney Injury.  |  Liang, NN., et al. 2024. Adv Sci (Weinh). 11: e2404753. PMID: 39303219
9. PACS2/CPT1A/DHODH signaling promotes cardiomyocyte ferroptosis in diabetic cardiomyopathy.  |  Xiang, H., et al. 2024. Cardiovasc Diabetol. 23: 432. PMID: 39633391
10. Targeting both the enzymatic and non-enzymatic functions of DHODH as a therapeutic vulnerability in c-Myc-driven cancer.  |  Zhang, Q., et al. 2025. Cell Rep. 44: 115327. PMID: 39977268

The preferred DHODH antibody is DHODH Antibody (E-8): sc-166348.