DGK-ι Antibody (G-3): sc-166439
- DGK-ι Antibody (G-3) is a mouse monoclonal IgG1 κ DGK-ι antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1017-1046 at the C-terminus of DGK-ι of human origin
- DGK-iota Antibody (G-3) is recommended for detection of DGK-ι of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DGK-iota Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DGK-ι Antibody (G-3) for WB applications. These reagents are now offered in bundles with DGK-ι Antibody (G-3) (see ordering information below).
DGK-ι Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects DGK-iota protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DGK-ι (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DGK-iota protein plays a crucial role in cellular signaling by phosphorylating diacylglycerol (DAG) to generate phosphatidic acid, which are both important lipid second messengers involved in various signaling pathways. This process is vital for regulating cell activation and secretion, influencing numerous physiological responses, including cell growth, differentiation, and apoptosis. Notably, DGK-iota is predominantly expressed in the brain and retina, where DGK-iota contributes to the modulation of neuronal signaling and synaptic plasticity. The presence of DGK-iota in these tissues underscores its significance in neurobiology and potential implications in neurodegenerative diseases. With DGK-iota′s ability to interact with other diacylglycerol kinases and various signaling molecules, DGK-iota serves as a key player in maintaining cellular homeostasis and responding to external stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DGK-ι Antibody (G-3) References:
- Mammalian diacylglycerol kinases, a family of lipid kinases with signaling functions. | Topham, MK. and Prescott, SM. 1999. J Biol Chem. 274: 11447-50. PMID: 10206945
- Purification, cDNA-cloning and expression of human diacylglycerol kinase. | Schaap, D., et al. 1990. FEBS Lett. 275: 151-8. PMID: 2175712
- Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons. | Goto, K. and Kondo, H. 1993. Proc Natl Acad Sci U S A. 90: 7598-602. PMID: 7689223
- Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells. | Kai, M., et al. 1994. J Biol Chem. 269: 18492-8. PMID: 8034597
- Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats. | Masai, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 11157-61. PMID: 8248222
- Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases. | Sakane, F., et al. 1996. J Biol Chem. 271: 8394-401. PMID: 8626538
- Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates. | Tang, W., et al. 1996. J Biol Chem. 271: 10237-41. PMID: 8626589
- Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase. | Klauck, TM., et al. 1996. J Biol Chem. 271: 19781-8. PMID: 8702685
- The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. | Ding, L., et al. 1998. J Biol Chem. 273: 32746-52. PMID: 9830018
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGK-ι Antibody (G-3) | sc-166439 | 200 µg/ml | $316.00 | |||
DGK-ι Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-528969 | 200 µg Ab; 10 µg BP | $354.00 | |||
DGK-ι Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-521610 | 200 µg Ab, 40 µg BP | $354.00 | |||
DGK-ι Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-543245 | 200 µg Ab; 20 µg BP | $354.00 | |||
DGK-ι Antibody (G-3) AC | sc-166439 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DGK-ι Antibody (G-3) HRP | sc-166439 HRP | 200 µg/ml | $316.00 | |||
DGK-ι Antibody (G-3) FITC | sc-166439 FITC | 200 µg/ml | $330.00 | |||
DGK-ι Antibody (G-3) PE | sc-166439 PE | 200 µg/ml | $343.00 | |||
DGK-ι Antibody (G-3) Alexa Fluor® 488 | sc-166439 AF488 | 200 µg/ml | $357.00 | |||
DGK-ι Antibody (G-3) Alexa Fluor® 546 | sc-166439 AF546 | 200 µg/ml | $357.00 | |||
DGK-ι Antibody (G-3) Alexa Fluor® 594 | sc-166439 AF594 | 200 µg/ml | $357.00 | |||
DGK-ι Antibody (G-3) Alexa Fluor® 647 | sc-166439 AF647 | 200 µg/ml | $357.00 | |||
DGK-ι Antibody (G-3) Alexa Fluor® 680 | sc-166439 AF680 | 200 µg/ml | $357.00 | |||
DGK-ι Antibody (G-3) Alexa Fluor® 790 | sc-166439 AF790 | 200 µg/ml | $357.00 | |||
DGK-ι (G-3) Neutralizing Peptide | sc-166439 P | 100 µg/0.5 ml | $68.00 |