DGK-α Antibody (C-11): sc-271644

DGK-α Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects DGK-alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-DGK-alpha antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The diacylglycerol kinase alpha (DGK-α) protein plays a crucial role in cellular signaling by phosphorylating diacylglycerol (DAG) to generate phosphatidic acid, which are both important lipid second messengers involved in various signaling pathways. This enzymatic activity is vital for regulating cell activation and the secretion of exosomes that can influence cell death, thereby impacting processes such as immune response and cancer progression. Additionally, DGK-α is known to interact with other proteins involved in signaling pathways, further emphasizing its importance in cellular communication and function. The expression of DGK-α is particularly notable in immune cells, where DGK-α modulates responses to external stimuli, making DGK-α a significant target for research in immunology and therapeutic interventions.

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DGK-α Antibody (C-11) References:

1. Mammalian diacylglycerol kinases, a family of lipid kinases with signaling functions.  |  Topham, MK. and Prescott, SM. 1999. J Biol Chem. 274: 11447-50. PMID: 10206945
2. Purification, cDNA-cloning and expression of human diacylglycerol kinase.  |  Schaap, D., et al. 1990. FEBS Lett. 275: 151-8. PMID: 2175712
3. Diacylglycerol Kinase alpha in X Linked Lymphoproliferative Disease Type 1.  |  Velnati, S., et al. 2021. Int J Mol Sci. 22: PMID: 34072296
4. Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma.  |  Liu, W., et al. 2024. Cancer Commun (Lond). 44: 226-250. PMID: 38143235
5. Tumor-associated macrophage (TAM)-secreted CCL22 confers cisplatin resistance of esophageal squamous cell carcinoma (ESCC) cells via regulating the activity of diacylglycerol kinase α (DGKα)/NOX4 axis.  |  Chen, J., et al. 2024. Drug Resist Updat. 73: 101055. PMID: 38387281
6. Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons.  |  Goto, K. and Kondo, H. 1993. Proc Natl Acad Sci U S A. 90: 7598-602. PMID: 7689223
7. Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells.  |  Kai, M., et al. 1994. J Biol Chem. 269: 18492-8. PMID: 8034597
8. Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats.  |  Masai, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 11157-61. PMID: 8248222
9. Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases.  |  Sakane, F., et al. 1996. J Biol Chem. 271: 8394-401. PMID: 8626538
10. Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates.  |  Tang, W., et al. 1996. J Biol Chem. 271: 10237-41. PMID: 8626589
11. Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase.  |  Klauck, TM., et al. 1996. J Biol Chem. 271: 19781-8. PMID: 8702685
12. The cloning and characterization of a novel human diacylglycerol kinase, DGKiota.  |  Ding, L., et al. 1998. J Biol Chem. 273: 32746-52. PMID: 9830018

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DGK-α Antibody (C-11) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DGK-α Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DGK-α Antibody (C-11) Specifications

Alternate Product Name: DGK-α

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 242-298

Epitope Target: DGK-α

Epitope Species: human

Product Reactivity/Detection: DGK-α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DGK-α Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin