desmoplakin I/II Antibody (A-1) recommended for detection of desmoplakin I/II of human origin by WB, IP, IF, IHC(P) and ELISA

desmoplakin I/II Antibody (A-1): sc-390975

desmoplakin I/II Antibody (A-1) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-desmoplakin I/II Antibody (A-1) is a mouse monoclonal IgG1 (kappa light chain) desmoplakin I/II antibody provided at 200 µg/ml
    • raised against amino acids 79-300 mapping near the N-terminus of desmoplakin I of human origin
    • desmoplakin I/II Antibody (A-1) is recommended for detection of desmoplakin I/II of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-desmoplakin I/II Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of desmoplakin I/II (A-1): sc-390975.
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Does this antibody work in canine cells (specifically MDCK)?

Asked by: Rutgers
Thank you for your question. This antibody is recommended for use on human samples only. It has not been tested on dog samples, and can not be recommended or warrantied for mouse samples.
Answered by: Tech Service
Date published: 2019-08-13

I am using desmoplakin I/II (A-1): sc-390975 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great monoclonal for WB and IF I used this antibody in ovarian cancer cells in EMT. It works very well. Good signal at the right size.Dilution used: (1/300 to 1/500)
Date published: 2018-11-22
Rated 5 out of 5 by from Didnot work for WB Produced distinct bands on WB. Used the antibody at 1:1000 dilution
Date published: 2017-07-03
Rated 5 out of 5 by from Great monoclonal for IHC and WB Clean specific staining on immunohistochemistry and nice solid bands on WB. Great choice!
Date published: 2017-01-20
Rated 5 out of 5 by from Grat feature used in western blot in prostate cancer cell lines in 1:250 dilution
Date published: 2016-11-19
Rated 5 out of 5 by from Superb immunoperoxidase membrane and cytoplasmic Superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Worked well in WB Worked well in WB!
Date published: 2015-01-03
Rated 5 out of 5 by from Satisfactory Western Blot data of desmoplakin Satisfactory Western Blot data of desmoplakin I/II expression in HeLa whole cell lysate. -SCBT QC
Date published: 2013-02-01
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