DEPTOR Antibody (A-3): sc-398169

DEPTOR Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects DEPTOR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DEPTOR antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DEPTOR, or DEP domain containing MTOR-interacting protein, is a 409 amino acid protein that plays a crucial role in the negative regulation of mTORC1 and mTORC2 signaling pathways, which are essential for cell growth, proliferation, and survival. DEPTOR′s ability to modulate these pathways is significant, as dysregulation can lead to various diseases, including cancer and metabolic disorders. DEPTOR interacts with FRAP protein through PDZ domain and undergoes post-translational modifications, including phosphorylation, which can influence activity and stability. DEPTOR gene is located on human chromosome 8q24.12, a region associated with numerous genetic disorders and malignancies, highlighting DEPTOR′s importance in cellular signaling and disease.

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DEPTOR Antibody (A-3) References:

1. Preferential expression of an intact WRN gene in Werner syndrome cell lines in which a normal chromosome 8 has been introduced.  |  Kashino, G., et al. 2001. Biochem Biophys Res Commun. 289: 111-5. PMID: 11708785
2. Cytogenetic mapping of a novel locus for type II Waardenburg syndrome.  |  Selicorni, A., et al. 2002. Hum Genet. 110: 64-7. PMID: 11810298
3. Combined analysis from eleven linkage studies of bipolar disorder provides strong evidence of susceptibility loci on chromosomes 6q and 8q.  |  McQueen, MB., et al. 2005. Am J Hum Genet. 77: 582-95. PMID: 16175504
4. Epigenetic inactivation of the premature aging Werner syndrome gene in human cancer.  |  Agrelo, R., et al. 2006. Proc Natl Acad Sci U S A. 103: 8822-7. PMID: 16723399
5. Non-Hodgkin's lymphomas with Burkitt-like cells are associated with c-Myc amplification and poor prognosis.  |  Mossafa, H., et al. 2006. Leuk Lymphoma. 47: 1885-93. PMID: 17065002
6. mTOR in renal cell cancer: modulator of tumor biology and therapeutic target.  |  Wysocki, PJ. 2009. Expert Rev Mol Diagn. 9: 231-41. PMID: 19379082
7. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival.  |  Peterson, TR., et al. 2009. Cell. 137: 873-86. PMID: 19446321
8. DEPTOR at the Nexus of Cancer, Metabolism, and Immunity.  |  Caron, A., et al. 2018. Physiol Rev. 98: 1765-1803. PMID: 29897294
9. DEPTOR stabilizes ErbB2 to promote the proliferation and survival of ErbB2-positive breast cancer cells.  |  Bi, Y., et al. 2021. Theranostics. 11: 6355-6369. PMID: 33995662
10. DEPTOR in Skeletal Development and Diseases.  |  Perez-Tejeiro, JM. and Csukasi, F. 2021. Front Genet. 12: 667283. PMID: 34122519
11. The UBE2C/CDH1/DEPTOR axis is an oncogene and tumor suppressor cascade in lung cancer cells.  |  Zhang, S., et al. 2023. J Clin Invest. 133: PMID: 36548081
12. PUM1 Promotes Tumor Progression by Activating DEPTOR-Meditated Glycolysis in Gastric Cancer.  |  Yin, S., et al. 2023. Adv Sci (Weinh). 10: e2301190. PMID: 37469018

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DEPTOR Antibody (A-3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-DEPTOR Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DEPTOR Antibody (A-3) Specifications

Alternate Product Name: DEPTOR

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 18-37

Epitope Target: DEPTOR

Epitope Species: human

Product Reactivity/Detection: DEPTOR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for DEPTOR Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin