DDX56 Antibody (F-5): sc-393078

DDX56 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDX56 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX56 Antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX56, also known as DDX21 or NOH61, plays a crucial role in ribosome synthesis, particularly in the assembly of the large 60S ribosomal subunit, which is essential for protein translation in cells. This process is vital for cellular function and growth, as ribosomes are the machinery that translates messenger RNA into proteins, thereby influencing various cellular activities and overall organismal development. DDX56 contains a helicase core region, a leucine zipper motif at its N-terminus, two putative C-terminal nuclear localization signals, and several potential phosphorylation sites, which may regulate activity and localization within the cell. These structural features highlight DDX56′s importance in maintaining proper ribosomal function and cellular homeostasis, making DDX56 a significant target for research in developmental biology and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DDX56 Antibody (F-5) References:

1. A novel helicase-type protein in the nucleolus: protein NOH61.  |  Zirwes, RF., et al. 2000. Mol Biol Cell. 11: 1153-67. PMID: 10749921
2. Silencing of RNA helicase II/Gualpha inhibits mammalian ribosomal RNA production.  |  Henning, D., et al. 2003. J Biol Chem. 278: 52307-14. PMID: 14559904
3. Molecular cloning and characterization of a putative nuclear DEAD box RNA helicase in the spruce budworm, Choristoneura fumiferana.  |  Zhang, DY., et al. 2006. Arch Insect Biochem Physiol. 61: 209-19. PMID: 16552767
4. The E. coli RhlE RNA helicase regulates the function of related RNA helicases during ribosome assembly.  |  Jain, C. 2008. RNA. 14: 381-9. PMID: 18083833
5. Cooperative binding of ATP and RNA induces a closed conformation in a DEAD box RNA helicase.  |  Theissen, B., et al. 2008. Proc Natl Acad Sci U S A. 105: 548-53. PMID: 18184816
6. Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev function.  |  Yasuda-Inoue, M., et al. 2013. Biochem Biophys Res Commun. 434: 803-8. PMID: 23608157
7. DDX3 RNA helicase is required for HIV-1 Tat function.  |  Yasuda-Inoue, M., et al. 2013. Biochem Biophys Res Commun. 441: 607-11. PMID: 24183723
8. Immune-Related RNA-Binding Protein-Based Signature With Predictive and Prognostic Implications in Patients With Lung Adenocarcinoma.  |  Xu, L., et al. 2022. Front Mol Biosci. 9: 807622. PMID: 35647031
9. DDX56 transcriptionally activates MIST1 to facilitate tumorigenesis of HCC through PTEN-AKT signaling.  |  Zhou, H., et al. 2022. Theranostics. 12: 6069-6087. PMID: 36168636
10. A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.  |  Py, B., et al. 1996. Nature. 381: 169-72. PMID: 8610017
11. Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family.  |  Imamura, O., et al. 1997. Biochem Biophys Res Commun. 240: 335-40. PMID: 9388478
12. A novel DEAD-box RNA helicase exhibits high sequence conservation from yeast to humans.  |  Eisen, A., et al. 1998. Biochim Biophys Acta. 1397: 131-6. PMID: 9565667

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX56 Antibody (F-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX56 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX56 Antibody (F-5) Specifications

Alternate Product Name: DDX56

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 373-514

Epitope Target: DDX56

Epitope Species: human

Product Reactivity/Detection: DDX56

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for DDX56 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent