DDX47 Antibody (H-9): sc-377333
- DDX47 Antibody (H-9) is a mouse monoclonal IgG2a κ DDX47 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 395-429 near the C-terminus of DDX47 of human origin
- DDX47 Antibody (H-9) is recommended for detection of DDX47 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DDX47 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX47 Antibody (H-9) for WB and IHC(P) applications. These reagents are now offered in bundles with DDX47 Antibody (H-9) (see ordering information below).
DDX47 Antibody (H-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects DDX47 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-DDX47 antibody (H-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX47, or probable ATP-dependent RNA helicase DDX47, is a 455 amino acid protein encoded by the human gene DDX47 and belongs to the DEAD-box helicase family, specifically the DDX47/RRP3 subfamily. DDX47 contains one helicase ATP-binding domain and one helicase C-terminal domain, which are crucial for RNA helicase function. DDX47 plays a key role in modulating RNA structure, essential for various biological processes such as transcription, splicing, and translation. Through nucleoside triphosphate hydrolysis, DDX47 facilitates RNA unwinding, influencing ribosome and spliceosome assembly. DDX47′s involvement in these critical cellular processes highlights its significance in embryogenesis, spermatogenesis, and cellular growth and division, making DDX47 a vital target for research in molecular biology and developmental studies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDX47 Antibody (H-9) References:
- The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus. | Doorbar, J., et al. 2000. J Virol. 74: 10081-95. PMID: 11024137
- Verge: a novel vascular early response gene. | Regard, JB., et al. 2004. J Neurosci. 24: 4092-103. PMID: 15102925
- RNA helicases: regulators of differentiation. | Abdelhaleem, M. 2005. Clin Biochem. 38: 499-503. PMID: 15885226
- GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47). | Lee, JH., et al. 2005. Biotechnol Lett. 27: 623-8. PMID: 15977068
- Phosphoproteome analysis of the human mitotic spindle. | Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
- NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47. | Sekiguchi, T., et al. 2006. Nucleic Acids Res. 34: 4593-608. PMID: 16963496
- 18S rRNA processing requires the RNA helicase-like protein Rrp3. | O'Day, CL., et al. 1996. Nucleic Acids Res. 24: 3201-7. PMID: 8774901
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX47 Antibody (H-9) | sc-377333 | 200 µg/ml | $322.00 | |||
DDX47 Antibody (H-9): m-IgGκ BP-HRP Bundle | sc-523286 | 200 µg Ab, 40 µg BP | $361.00 | |||
DDX47 Antibody (H-9): m-IgG2a BP-HRP Bundle | sc-546152 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDX47 Antibody (H-9) AC | sc-377333 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DDX47 Antibody (H-9) HRP | sc-377333 HRP | 200 µg/ml | $322.00 | |||
DDX47 Antibody (H-9) FITC | sc-377333 FITC | 200 µg/ml | $336.00 | |||
DDX47 Antibody (H-9) PE | sc-377333 PE | 200 µg/ml | $349.00 | |||
DDX47 Antibody (H-9) Alexa Fluor® 488 | sc-377333 AF488 | 200 µg/ml | $364.00 | |||
DDX47 Antibody (H-9) Alexa Fluor® 546 | sc-377333 AF546 | 200 µg/ml | $364.00 | |||
DDX47 Antibody (H-9) Alexa Fluor® 594 | sc-377333 AF594 | 200 µg/ml | $364.00 | |||
DDX47 Antibody (H-9) Alexa Fluor® 647 | sc-377333 AF647 | 200 µg/ml | $364.00 | |||
DDX47 Antibody (H-9) Alexa Fluor® 680 | sc-377333 AF680 | 200 µg/ml | $364.00 | |||
DDX47 Antibody (H-9) Alexa Fluor® 790 | sc-377333 AF790 | 200 µg/ml | $364.00 | |||
DDX47 (H-9) Neutralizing Peptide | sc-377333 P | 100 µg/0.5 ml | $69.00 |