DDX11 Antibody (D-2): sc-271711
- DDX11 Antibody (D-2) is a mouse monoclonal IgG2b κ DDX11 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 405-704 mapping within an internal region of DDX11 of human origin
- DDX11 Antibody (D-2) is recommended for detection of DDX11 of human origin by WB, IP, IF and ELISA
- Anti-DDX11 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX11 Antibody (D-2) for WB applications. These reagents are now offered in bundles with DDX11 Antibody (D-2) (see ordering information below).
DDX11 Antibody (D-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects DDX11 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX11 Antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX11, also known as CHLR1 or KRG2, belongs to the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp motif and functions as an RNA helicase. DDX11 plays a crucial role in several cellular processes, including RNA secondary structure modifications and assembly of ribosomes and spliceosomes. DDX11 localizes primarily in the nucleus and shows high expression in tissues such as testis, thymus, ovary, spleen, and pancreas, highlighting DDX11′s importance in cellular growth and division. DDX11 is essential for proper chromosome segregation and embryonic development, with binding capability to both single- and double-stranded DNA. Five isoforms of DDX11, resulting from alternative splicing, underscore DDX11′s complexity and regulatory potential in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDX11 Antibody (D-2) References:
- Characterization of the enzymatic activity of hChlR1, a novel human DNA helicase. | Hirota, Y. and Lahti, JM. 2000. Nucleic Acids Res. 28: 917-24. PMID: 10648783
- Radiation hybrid mapping of 18 positional and physiological candidate genes for arthrogryposis multiplex congenita on porcine chromosome 5. | Genini, S., et al. 2006. Anim Genet. 37: 239-44. PMID: 16734683
- The consensus coding sequences of human breast and colorectal cancers. | Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
- The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. | Parish, JL., et al. 2006. J Cell Sci. 119: 4857-65. PMID: 17105772
- ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance. | Parish, JL., et al. 2006. Mol Cell. 24: 867-76. PMID: 17189189
- Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation. | Inoue, A., et al. 2007. Cell Cycle. 6: 1646-54. PMID: 17611414
- The human homologue of the yeast CHL1 gene is a novel keratinocyte growth factor-regulated gene. | Frank, S. and Werner, S. 1996. J Biol Chem. 271: 24337-40. PMID: 8798685
- Localization of chi1-related helicase genes to human chromosome regions 12p11 and 12p13: similarity between parts of these genes and conserved human telomeric-associated DNA. | Amann, J., et al. 1996. Genomics. 32: 260-5. PMID: 8833153
- Characterization of putative human homologues of the yeast chromosome transmission fidelity gene, CHL1. | Amann, J., et al. 1997. J Biol Chem. 272: 3823-32. PMID: 9013641
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX11 Antibody (D-2) | sc-271711 | 200 µg/ml | $316.00 | |||
DDX11 Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-529248 | 200 µg Ab; 10 µg BP | $354.00 | |||
DDX11 Antibody (D-2): m-IgGκ BP-HRP Bundle | sc-522035 | 200 µg Ab, 40 µg BP | $354.00 | |||
DDX11 Antibody (D-2): m-IgG2b BP-HRP Bundle | sc-550086 | 200 µg Ab; 10 µg BP | $354.00 | |||
DDX11 Antibody (D-2) AC | sc-271711 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DDX11 Antibody (D-2) HRP | sc-271711 HRP | 200 µg/ml | $316.00 | |||
DDX11 Antibody (D-2) FITC | sc-271711 FITC | 200 µg/ml | $330.00 | |||
DDX11 Antibody (D-2) PE | sc-271711 PE | 200 µg/ml | $343.00 | |||
DDX11 Antibody (D-2) Alexa Fluor® 488 | sc-271711 AF488 | 200 µg/ml | $357.00 | |||
DDX11 Antibody (D-2) Alexa Fluor® 546 | sc-271711 AF546 | 200 µg/ml | $357.00 | |||
DDX11 Antibody (D-2) Alexa Fluor® 594 | sc-271711 AF594 | 200 µg/ml | $357.00 | |||
DDX11 Antibody (D-2) Alexa Fluor® 647 | sc-271711 AF647 | 200 µg/ml | $357.00 | |||
DDX11 Antibody (D-2) Alexa Fluor® 680 | sc-271711 AF680 | 200 µg/ml | $357.00 | |||
DDX11 Antibody (D-2) Alexa Fluor® 790 | sc-271711 AF790 | 200 µg/ml | $357.00 |