DcpS Antibody (A-12): sc-393226
- DcpS Antibody (A-12) is a mouse monoclonal IgG2b κ DcpS antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 97-124 within an internal region of DcpS of human origin
- DcpS Antibody (A-12) is recommended for detection of DcpS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and porcine
- Anti-DcpS Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DcpS Antibody (A-12) for WB and IHC(P) applications. These reagents are now offered in bundles with DcpS Antibody (A-12) (see ordering information below).
QUICK LINKS
SEE ALSO...
DcpS Antibody (A-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects DcpS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DcpS (A-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DcpS plays a crucial role in mRNA degradation, specifically functioning as a scavenger pyrophosphatase that hydrolyzes the residual cap structure after the 3′ to 5′ decay of mRNA. This process is vital for cellular regulation of gene expression and the recycling of nucleotides, as DcpS releases N-7 methyl guanosine monophosphate and 5′-diphosphate terminated cap products following mRNA degradation. The central histidine within the DcpS HIT motif is essential for decapping activity, establishing the HIT motif as a novel mRNA decapping domain and positioning DcpS as the first member of the HIT protein family with a defined biological function. Notably, HIT proteins are characterized by their homodimeric structure and contain two conserved 100-amino-acid HIT fold domains, each with independent active sites capable of binding and hydrolyzing their respective substrates, underscoring the importance of DcpS in mRNA metabolism and cellular homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DcpS Antibody (A-12) References:
- Functional link between the mammalian exosome and mRNA decapping. | Wang, Z. and Kiledjian, M. 2001. Cell. 107: 751-62. PMID: 11747811
- The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases. | Liu, H., et al. 2002. EMBO J. 21: 4699-708. PMID: 12198172
- The hDcp2 protein is a mammalian mRNA decapping enzyme. | Wang, Z., et al. 2002. Proc Natl Acad Sci U S A. 99: 12663-8. PMID: 12218187
- Insights into the structure, mechanism, and regulation of scavenger mRNA decapping activity. | Gu, M., et al. 2004. Mol Cell. 14: 67-80. PMID: 15068804
- Diagnosis of sinusitis in children: emphasis on the history and physical examination. | Fireman, P. 1992. J Allergy Clin Immunol. 90: 433-6. PMID: 1527332
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DcpS Antibody (A-12) | sc-393226 | 200 µg/ml | $322.00 | |||
DcpS Antibody (A-12): m-IgG Fc BP-HRP Bundle | sc-530396 | 200 µg Ab; 10 µg BP | $361.00 | |||
DcpS Antibody (A-12): m-IgGκ BP-HRP Bundle | sc-523861 | 200 µg Ab, 40 µg BP | $361.00 | |||
DcpS Antibody (A-12): m-IgG2b BP-HRP Bundle | sc-550187 | 200 µg Ab; 10 µg BP | $361.00 | |||
DcpS Antibody (A-12) AC | sc-393226 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DcpS Antibody (A-12) HRP | sc-393226 HRP | 200 µg/ml | $322.00 | |||
DcpS Antibody (A-12) FITC | sc-393226 FITC | 200 µg/ml | $336.00 | |||
DcpS Antibody (A-12) PE | sc-393226 PE | 200 µg/ml | $349.00 | |||
DcpS Antibody (A-12) Alexa Fluor® 488 | sc-393226 AF488 | 200 µg/ml | $364.00 | |||
DcpS Antibody (A-12) Alexa Fluor® 546 | sc-393226 AF546 | 200 µg/ml | $364.00 | |||
DcpS Antibody (A-12) Alexa Fluor® 594 | sc-393226 AF594 | 200 µg/ml | $364.00 | |||
DcpS Antibody (A-12) Alexa Fluor® 647 | sc-393226 AF647 | 200 µg/ml | $364.00 | |||
DcpS Antibody (A-12) Alexa Fluor® 680 | sc-393226 AF680 | 200 µg/ml | $364.00 | |||
DcpS Antibody (A-12) Alexa Fluor® 790 | sc-393226 AF790 | 200 µg/ml | $364.00 | |||
DcpS (A-12) Neutralizing Peptide | sc-393226 P | 100 µg/0.5 ml | $69.00 |