DAP-5 Antibody (F-2): sc-137011

DAP-5 Antibody (F-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects DAP-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DAP-5 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Death-associated protein 5 (DAP-5), also known as p97 and NAT1, is a crucial member of the eukaryotic translation initiation factor-4G (eIF4G) family, playing a significant role in protein synthesis regulation. DAP-5 is ubiquitously expressed and highly conserved across species, which underscores its importance in cellular functions. When activated by FAS or p53, DAP-5 undergoes caspase cleavage at position 790, resulting in a C-terminal truncated form that can form complexes with eIF4A and eIF3. This truncated protein lacks the N-terminal region of eIF4G that binds to the cap-binding protein eIF4E, allowing DAP-5 to act as a general repressor of translation by forming inactive complexes. During apoptosis, the caspase-activated DAP-5 can mediate cap-independent translation from its own internal ribosome entry site, creating a positive feedback loop that sustains DAP-5 translation. DAP-5 is essential for cellular differentiation, as DAP-5 regulates specific gene expression pathways, highlighting its multifaceted role in both normal cellular processes and stress responses.

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DAP-5 Antibody (F-2) References:

1. A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation.  |  Henis-Korenblit, S., et al. 2000. Mol Cell Biol. 20: 496-506. PMID: 10611228
2. Essential role of NAT1/p97/DAP5 in embryonic differentiation and the retinoic acid pathway.  |  Yamanaka, S., et al. 2000. EMBO J. 19: 5533-41. PMID: 11032820
3. DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells.  |  Wittke, I., et al. 2001. Cancer Lett. 162: 237-43. PMID: 11146231
4. The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins.  |  Henis-Korenblit, S., et al. 2002. Proc Natl Acad Sci U S A. 99: 5400-5. PMID: 11943866
5. DAP5 and IRES-mediated translation during programmed cell death.  |  Marash, L. and Kimchi, A. 2005. Cell Death Differ. 12: 554-62. PMID: 15818401
6. A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme.  |  Yamanaka, S., et al. 1997. Genes Dev. 11: 321-33. PMID: 9030685
7. DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death.  |  Levy-Strumpf, N., et al. 1997. Mol Cell Biol. 17: 1615-25. PMID: 9032289
8. A new translational regulator with homology to eukaryotic translation initiation factor 4G.  |  Imataka, H., et al. 1997. EMBO J. 16: 817-25. PMID: 9049310
9. Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions.  |  Levy-Strumpf, N. and Kimchi, A. 1998. Oncogene. 17: 3331-40. PMID: 9916995

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAP-5 Antibody (F-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DAP-5 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAP-5 Antibody (F-2) Specifications

Alternate Product Name: DAP-5

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 608-907

Epitope Target: DAP-5

Epitope Species: human

Product Reactivity/Detection: DAP-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DAP-5 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin