DAP-3 Antibody (E-9): sc-398441

DAP-3 Antibody (E-9) is a mouse monoclonal IgG2b antibody that detects DAP-3 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP-3 is a crucial nucleotide-binding protein that plays a significant role in the regulation of programmed cell death, making it essential for maintaining cellular homeostasis and preventing uncontrolled cell proliferation. DAP-3 is characterized by its potential P-loop motif, which is vital for its nucleotide-binding capabilities. DAP-3 functions downstream of receptor signaling complexes, and pro-apoptotic effects are contingent upon the activity of caspases, which are key enzymes in the apoptosis pathway. Notably, DAP-3 interacts with the glucocorticoid receptor, specifically through its amino-terminal region, which acts in a dominant-negative manner to shield cells from apoptosis, thereby highlighting its dual role in cell survival and death. DAP-3 is conserved across species at both functional and structural levels and is widely expressed in highly proliferative epithelial tissues, underscoring its importance in various biological processes. Uniquely, DAP-3 maintains its localization in the mitochondria even during apoptosis, distinguishing DAP-3 from many other apoptotic regulators and emphasizing its critical role in the apoptotic signaling cascade.

The preferred DAP-3 antibody is DAP-3 Antibody (F-12): sc-373911.